江苏省发热伴血小板减少综合征布尼亚病毒的核酸检测及全基因组序列分析  

Detection of RNA and analysis of complete genome of severe fever with thrombocytopenia syndrome virus (SFTSV) in Jiangsu

作　　者：单军[1] 唐震[1] 崔仑标[1] 祁贤[1] 郭喜玲[1] 赵康辰[1] 葛以跃[1] 单云峰[1] 史智扬[1] 鲍倡俊[1] 汤奋扬[1] 祖荣强[1] 

机构地区：[1]江苏省疾病预防控制中心,江苏南京210009

出　　处：《现代预防医学》2013年第14期2686-2689,2696,共5页Modern Preventive Medicine

基　　金：江苏省临床医学中心(高技术平台);ZX201109;江苏省医学重点人才基金;RC2011084

摘　　要：目的对江苏省2010年发热伴血小板减少综合征布尼亚病毒(SFTSV)进行核酸检测并分析其全基因组序列。方法用荧光定量PCR的方法对江苏省2010年SFTSV感染病例进行核酸检测,将所有阳性标本进行病毒分离,取8株代表性毒株测定其全基因组序列,序列结果用DNAStar和MEGA4软件进行分析。结果 33例发热伴血小板减少综合征疑似病例中,20例SFTSV核酸检测阳性。1例狗标本SFTSV检测也为阳性。8株SFTSV毒株全基因组序列与国内其他地区流行株的序列高度同源,其中M片段核苷酸序列同源性介于95.8%～98.7%之间,氨基酸序列同源性介于98.7%～99.7%之间;而与汉坦病毒属和白蛉热病毒属的相关序列相比差异较大,M段核苷酸序列同源性最高仅为27.1%,氨基酸序列同源性最高仅为7.3%。与一代病例密切接触后发病的二代病例序列与一代病例序列完全一致;狗携带的SFTSV序列与病人序列高度同源。结论 SFTSV江苏株与国内参考株序列无明显差异,与布尼亚病毒科的其他属序列差异巨大;SFTSV存在人传人的可能性;狗可能是SFTSV的传播宿主之一。OBJECTIVE To detect RNA and analyze complete genome of severe fever with thrombocytopenia syndrome virus （SFTSV） in Jiangsu Province. METHODS Real-time RT-PCR was used to detect RNA of SFTSV in Jiangsu Province 2010. All positive samples were carried on the simple isolated culture to the virus and 8 strains were selected to sequence complete genome, analyzed by software DNA Star and MEGA4. RESULTS 20 cases of human SFTSV infection were confirmed by RT-PCR in 33 doubtful cases besides one positive case of dog. The sequence analysis showed that the homogeneity of M segment shared 95.8%-98.7% nucleotide acid identity, 98.7%-99.7% amino acid identity between these 8 strains and the representative strains of SFTSV isolated from other provinces in China. There were only maximum 27.1% nucleotide acid identity and maximum 7.3% amino acid identity among these 8 strains and the representative strains of Hantavirus and Phlebovirus. The sequence of secondary case was virtually identical with that of primary case. There was a high sequence homogeneity between the dog and the patients. CONCLUSION There is no apparent difference of sequence between Jiangsu Province and other Provinces in China. There exists a huge difference of sequence between SFTSV and other Bunyavirus. SFTSV of person-to-person contact is highly suggestive. The dog is probably one of animal hosts of SFTSV transmission.

关 键 词：发热伴血小板减少综合征布尼亚病毒 荧光定量PCR 序列比对 

分 类 号：R373.3[医药卫生—病原生物学]