白介素11及其受体在肝细胞癌组织表达及意义研究  

Expression and significance of interleukin-11(IL-11) and interleukin-11 receptor (IL-11R) in primary hepatic carcinoma

作　　者：孙传政[1] 黄飞舟[1] 杨明施[2] 蔡立峰[1] 邓刚[1] 刘怀政[2] 

机构地区：[1]中南大学湘雅三医院普外科,湖南长沙410013 [2]中南大学湘雅三医院急诊科,湖南长沙410013

出　　处：《中国实用外科杂志》2013年第7期584-586,共3页Chinese Journal of Practical Surgery

摘　　要：目的探讨白介素11(IL-11)、白介素11受体(IL-11R)与肝癌及相关临床和病理指标间的关系。方法收集2006年12月至2007年3月中南大学湘雅三医院普通外科30例肝细胞癌手术病人的新鲜肿瘤组织、邻近的非癌肝组织标本及其临床、病理资料。采用Westernblot法检测IL-11、IL-11R在肝癌组织、邻近的非癌肝组织中的表达。分别测定IL-11、IL-11R及β-actin带的积分光密度(IOD)值,以目的蛋白与相应β-actin的IOD值比值(A值)来表示目的蛋白的表达水平。结果 IL-11、IL-11R在肝癌组织中表达水平显著高于癌旁肝组织,IL-Ⅱ的表达与肿瘤的病理分级、包膜是否受侵犯、门静脉有无癌栓相关;IL-11R的表达与肿瘤有无淋巴结转移、包膜是否受侵犯、门静脉有无癌栓相关。结论 IL-11/IL-11R有可能在肝癌的发生、分化、侵袭及转移过程中发挥重要作用。Objective To clarify the role of IL-11 and IL-11R in human hepatocellular carcinoma. Methods Thirty primary hepatic carcinoma （PHC） tissues, their pericarcinomatous liver tissues and their clinicopathological factors admitted between December 2006 and March 2007 in the Third Affiliated Hospital, Central South University were obtained in the current study. Expression levers of IL-11 and IL-11R proteins in samples of pericarcinomatous liver and tumor tissue were analyzed by Western blot. The IOD of IL-11, IL-11R and β-actin was analyzed quantitatively by analysis software. The expression level of IL-11 and IL-11R was denoted by the ratio of IOD, which was called value A. Results The expression of IL-11 and IL-11R was upregulated obviously in PHC tissue. The expression of IL-11 was significantly related to Edmondson’s grade, capsule invasion and tumor’s thrombus in portal vein and the expression of IL-11R was correlated with Edmondson’s grad, capsule invasion and tumor’s thrombus in portal vein. Conclusion The finding suggests that IL-11/IL-11R may play important role in the occurrence, differentiation, invasion and transfer of PHC.

关 键 词：肝细胞癌 白介素11 白介素11受体 

分 类 号：R6[医药卫生—外科学]