HPLC测定九味双解口服液中大黄素的含量  

Determination of Rhein in Jiuwei Shuangjie Mixture by HPLC

作　　者：冷玉杰[1] 马艺璇[1] 沙东旭[2] 

机构地区：[1]辽宁中医药大学附属医院,辽宁沈阳110032 [2]辽宁省食品药品检验所,辽宁沈阳110023

出　　处：《辽宁中医杂志》2013年第7期1427-1430,共4页Liaoning Journal of Traditional Chinese Medicine

摘　　要：目的:建立HPLC法测定九味双解口服液中大黄素的含量。方法:以Dikma Diamonsil C18(200 mm×4.6 mm,5μm)为分析柱,甲醇-0.1%磷酸(85∶15)为流动相,流速为0.8 mL.min-1,检测波长为254 nm,采用外标法定量测定。结果:大黄素进样量在0.01682～0.4205μg范围内与峰面积呈良好的线性关系(r=0.99998)。平均回收率为103.5%;RSD为1.92%(n=6)。结论:本方法操作简便,结果准确可靠,适用于九味双解口服液中大黄素含量的测定。Objective：To establish an HPLC method for determination of Rhein in Jiuwei Shuangjie Mixture.Methods：Dikma Diamonsil C18 column（200 mm×4.6 mm,5 μm）was used,and the mobile phase consisted of MeOH-0.1%H3PO4（85∶ 15）.The flow rate was 0.8 mL·min-1 and the determination wavelength was at 254 nm.Results：The linear range was in the range of 0.01682~0.4205 μg（r=0.99998）.The average recovery was 103.5%;RSD=1.92%（n=6）.Conclusion：This method is simple,rapid and reliable for determination of Rhein in Jiuwei Shuangjie Mixture.

关 键 词：高效液相色谱 九味双解口服液 大黄素 含量测定 

分 类 号：R927.2[医药卫生—药学]