益肾达络饮对实验性自身免疫性脑脊髓炎小鼠神经功能评分和病理特征的影响  

Effect of Yishen Daluo Decoction on Score of Neural Function and Pathological Feature in Mice with Experimental Autoimmune Encephalomyelitis

作　　者：朱文浩[1] 高颖[1] 关东升[2] 吴彦青[3] 

机构地区：[1]北京中医药大学东直门医院,北京100700 [2]河南中医学院,河南郑州450003 [3]天津中医药大学,天津300193

出　　处：《辽宁中医杂志》2013年第7期1467-1469,I0002,共4页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金资助课题(81072770;81202657)

摘　　要：目的:研究益肾达络饮对复发-缓解型EAE小鼠发病率、发病时间、复发率、神经功能评分及病理特征的影响。方法:用HE染色和Luxol fast blue染色观察EAE小鼠的病理特征。结果:中药组EAE小鼠的复发率(14.27%)低于模型组(62.5%);免疫后第21天神经功能评分,中药组(1.86±0.40)低于模型组(3.13±0.69);免疫后第61天神经功能评分,中药组(0.86±0.55)低于模型组(2.75±0.73),差异有统计学意义(P<0.05)。结论:益肾达络饮能降低EAE小鼠的复发率、神经功能评分,减轻炎性细胞浸润和髓鞘脱失,但其作用机制需要进一步研究。Objective：To investigate the effect of Yishen Daluo Decoction on the incidence, onset, relapse rate, score of neural function and pathological feature in the mice with relapsing - remitting experimental autoimmune encephalomyelitis. Methods ： He- matoxylin - eosin staining and Luxol fast blue staining were used to observe the pathological feature in the mice with experimental autoimmune encephalomyelitis. Results： The relapse rate of the treated group （14.27%） was less than the model group＇s （62.5%） ;the score of neural function of the treated group（ 1.86 ±0.40）was less than the model group＇s （3.13 ±0.69） at the day 21 after immunization; the score of neural function of the treated group （0.86 ±0.55 ）was less than the model group＇s （2.75 ±0.73 ） at the day 61 after immunization （ P 〈 0. 05 ）. Conclusion ： After treatment with Yishen Daluo Decoction, the relapse rate and the score of neural function of the mice with experimental autoimmune encephalomyelitis are reduced, the infiltration of inflammatory cells and the loss of myelin are alleviated, but the effective mechanism of Yishen Daluo Decoction needs to be further explored.

关 键 词：益肾达络饮 实验性自身免疫性脑脊髓炎 神经功能评分 病理特征 

分 类 号：R373.31[医药卫生—病原生物学]