切断迷走神经对艾灸调控实验性类风湿性关节炎家兔关节滑膜液细胞因子水平的影响  

Effect of Vagotomy on Cytokine Level in RA Rabbits′ Synovial Fluid by Moxibustion

作　　者：张耀[1] 刘旭光[1] 杨涵棋[1] 杨馨[1] 王兴丽[1] 钟振东[2] 

机构地区：[1]山西中医学院,山西太原030024 [2]四川省人民医院实验动物研究所,四川成都610072

出　　处：《辽宁中医杂志》2013年第7期1482-1484,共3页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金资助课题(81173319)

摘　　要：目的:探索艾灸对实验性RA家兔关节滑膜液细胞因子水平的影响及其胆碱能神经调控机制。方法:将日本大耳白兔随机分为空白对照组、RA模型组、艾灸治疗组、艾灸治疗迷走神经切断组、艾灸治疗假手术组,用福氏完全佐剂塑造RA疾病模型,运用颈部左侧迷走神经干切断术作为干预手段,艾灸肾俞、足三里治疗,采用ELISA法检测滑膜液TNF-α、IL-1、IL-6、HMGB1含量。结果:与空白对照组相比较,RA模型组关节滑膜液中TNF-α、IL-1、IL-6、HMGB1含量显著增高(P<0.01);与RA模型组比较,艾灸治疗组、艾灸治疗假手术组关节滑膜液中TNF-α、IL-1、IL-6、HMGB1含量明显降低(P<0.01),而艾灸治疗迷走神经切断组无明显降低(P>0.05);与艾灸治疗组比较,艾灸治疗迷走神经切断组滑膜液中TNF-α、IL-1、IL-6、HMGB1含量显著增高(P<0.01),但艾灸治疗假手术组无明显增高(P>0.05)。结论:艾灸能抑制实验性RA家兔关节滑膜液促炎细胞因子水平从而减轻RA滑膜炎症,其机制可能与兴奋胆碱能抗炎通路有关。Objective：To explore the effect of cytokine level in RA rabbits＇synovial fluid by moxibustion and regulatory mecha- nism of cholinergic nerves. Methods ：Japan big-eared white rabbits were randomly divided into control group, model group, moxi- bustion treatment group, moxibustion treatment of vagotomy group, moxibustion treatment of sham operation group. RA animal mod- els were produced by Freunds＇Complete Adjuvant（FCA） and left truncal vagotomy of the neck was as a means of intervention, moxibusting in ＂Shenshu （ BL23 ） ＂ and ＂Zusanli （ 5T36 ） ＂ acupoints. TNF - α, IL - 1, IL - 6, HMGB1 in synovial fluid were de- tected by enzyme -linked immunosorbent assay （ELISA）. Results：Compared with the control group, RA model group＇s expressions of TNF-α, IL - 1, IL - 6, HMGB1 in synovial fluid were significantly increased（ P 〈 0. 01 ）. Compared with model group, moxibus- tion group and sham operation group were decreased significantly（ P 〈0.01 ）and vagotomy group was not decreased significantly （P 〉0. 05 ）. Compared with moxibustion group, vagotomy group were significantly increased（ P 〈 0. 01 ）, but the sham operation group was not significantly increased（ P 〉 0. 05 ）. Conclusion：Moxibustion can inhibit pro-inflammatory cytokine level in RA rab- bits＇synovial fluid and reduce the RA synovial membrane inflammation. Its mechanism may be related to activating cholinergic an- ti-inflammatory pathway.

关 键 词：胆碱能抗炎通路 艾灸 类风湿性关节炎 滑膜液 细胞因子 

分 类 号：R245.81[医药卫生—针灸推拿学]