耐低温贫营养好氧反硝化菌群脱氮特性及安全性  

Denitrification characters and safety of communities of cold tolerant oligotrophic and aerobic denitrifying bacteria

作　　者：黄廷林[1] 李娜[1] 张海涵[1] 王堃[1] 刘婷婷[1] 

机构地区：[1]西安建筑科技大学环境与市政工程学院,西安710055

出　　处：《环境工程学报》2013年第7期2419-2423,共5页Chinese Journal of Environmental Engineering

基　　金：国家科技支撑计划项目(2012BAC04B02);国家自然科学基金重点项目(50830303);陕西省科技统筹创新计划项目(2011KTCG03-07)

摘　　要：针对微污染水体强化原位生物脱氮技术同时面临低温、贫营养及好氧问题,对实验室已分离筛选的贫营养好氧反硝化菌和耐低温好氧反硝化菌进行菌源重组,构建出高效耐低温贫营养好氧脱氮功能菌群T1(Y3+F3+H8)和T2(Y3+F4)。研究不同投菌量条件下菌群的脱氮特性,结果表明,投菌量对T1脱氮效果有一定影响,0.1、0.2和1.0 mg/L投量对NO3--N去除率为71%、91%和100%,总氮去除率为56%、34%和52%;T2菌群,当投量为0.2 mg/L时,对NO3--N、总氮去除率最大可达66%和59.48%。对菌群T1、T2进行生物安全性分析,采用次氯酸钠进行消毒,其生物灭活率均达到99.9%以上。While being adopted to dispose micro polluted water, in situ biological denitrification will meet the problems, low temperature, oligotrophic and aerophile. The cold tolerant oligotrophic and aerobic denitrifying bacteria, isolated in the lab, were recombined to form the communities of T1 （Y3＋F3＋H8） and T2 （Y3＋F4）. Meanwhile, the denitrificaton characters of the two communities were investigated while the communities were employed for micro polluted water treatment at different bacterium dosages. The results showed that community T1 had different denitrifying capacities at the different bacterium dosages, denitrifying 71%, 91% and 100% of NO3--N, and 56%, 34% and 52% of total nitrogen at the bacterium dosages of 0.1 mg/L, 0.2 mg/L and 1.0 mg/L, respectively. Community T2 denitrified at most 66% and 59.48% of NO3--N and total nitrogen at the bacterium dosage of 0.2 mg/L, respectively. Sodium hypochlorite was found able to deactivate the bacteria of T1 and T2 at a percentage above 99.9%.

关 键 词：菌源重组 耐低温贫营养 好氧反硝化 投菌量 安全性 

分 类 号：X703[环境科学与工程—环境工程]