基因芯片技术及其在血液学和肿瘤学应用的前景  

The Gene Chip Technology and the Prospects of Its Application in Hematology and Oncology

作　　者：张新[1] 朱平[1] 

机构地区：[1]北京医科大学第一医院血液内科,北京100034

出　　处：《中国实验血液学杂志》2000年第3期222-226,共5页Journal of Experimental Hematology

摘　　要：介绍基因芯片的基本原理、制备的技术流程和不同的类型 ,在实际应用中基因芯片上的探针选择 ,受检标本的处理与杂交 ,在血液、肿瘤和细胞分化研究中应用基因芯片的情况 ,包括如何检测癌基因、抑癌基因以及细胞分化和凋亡基因的表达 ,分析人类基因的多态性与疾病易感性的关系。最后 。The basic principle, technological procedure and types of gene chips were introduced in the article. The probe choice in practical application and processing and hybridization of detected samples were described. Some researchers have used gene chips in hematology, oncology and cellular differentiation. Gene chips can be used to detect the expression of oncogenes, tumor suppression genes, and cell differentiation- and apoptosis-related genes. It can also help us to further study the association between the polymorphism of human genes and disease susceptibility. The aspects of development and problems in gene chips study were discussed.

关 键 词：基因芯片 DNA微列阵 探针 血液学 肿瘤学 

分 类 号：R552.03[医药卫生—血液循环系统疾病] R730.3[医药卫生—内科学]