鲤鱼精子染色方法学研究  

A study on different staining methods to Cyprinus carpio spermatozoon

作　　者：王建福[1] 李洁[1] 邵淑娟[1] 张勤[1] 张兆旺[1] 

机构地区：[1]甘肃农业大学动物科学技术学院,甘肃兰州730070

出　　处：《甘肃农业大学学报》2013年第3期19-23,共5页Journal of Gansu Agricultural University

基　　金：甘肃农业大学动物科学技术学院2010年青年教师科研基金项目

摘　　要：用龙胆紫、碱性复红、吉姆萨、甲基绿、苏木精-伊红Y、伊红Y-乙醇、伊红Y-水、伊红Y-苯胺黑等染液对鲤鱼精子进行染色,筛选适宜的鲤鱼精子染色方法.结果表明:采用不干燥固定直接伊红Y-乙醇压片染色的效果最好,染色清晰,精子头尾结构完整;自然干燥固定后再染色,可使鲤鱼精子头膜破裂细胞质漏出,致使精子染色形态模糊不清;4%的甲醛固定后再经自然干燥染色,可以有效避免鲤鱼精子头膜破裂;用1%的NaCl稀释的精液染色后,可清楚地观察到精子形态并区分出畸形精子,用水稀释的精液染色后可观察精子尾膜的完整性;体外活体伊红Y-苯胺黑染色法可以观察出精子头膜的完整性以及精子的损伤程度.To select a appropriate method of staining CyDrinus carpio spermatozoon, gentian violet, basic fuehsin,Giemsa, methyl green, hematoxylin eosin Y, eosin Y-ethanol, eosin Y-water, eosin Y-aniline black dye liquors were applied. The results showed that tabletting the spermatozoon with eosin Y before drying was the best method, the staining was clear, sperm was integrity. Staining after natural drying could make Cyprinus carpio spermatozoon^s head membrane rupture cytoplasmic leakage, resulting in sperm staining patterns fuzzy and incomplete. 4% formaldehyde fixed before natural drying could avoid carp sperm head membrane rupture. With 1% NaCl diluted semen staining could be clearly observed that the sperm morphology and distinguish abnormal sperm,diluted semen staining could be observed in sperm tail membrane integrity. A supravital eosin Y-aniline dyeing method could be observed head membrane integrity and damage degree of the sperm.

关 键 词：鲤鱼精子 干燥固定 染色 形态观察 

分 类 号：S917.4[农业科学—水产科学]