人胎盘AC133^+造血干/祖细胞的集落形成能力  

Capabilities of AC133^+ Cells,Forming Colony Derived from Human Placenta Tissues

作　　者：喻晓丹[1] 

机构地区：[1]遵义师范学院生命科学学院,贵州遵义563002

出　　处：《遵义师范学院学报》2013年第3期83-86,共4页Journal of Zunyi Normal University

摘　　要：探究了人体胎盘组织AC133+造血干/祖细胞(HSPC)的集落形成能力。选用新鲜的人胎盘组织制成单个细胞(有核细胞)悬液,经流式细胞术(FCM)分析其AC133+造血干/祖细胞的纯度,采用免疫磁性分选法(MACS)分选AC133+和AC133-HSPC,分别进行粒-单集落形成单位(CFU-GM)、红系集落形成单位(CFU-E)、混合集落形成单位(CFU-Mix)培养,以评价其增殖分化能力。结果显示胎盘组织AC133+细胞百分率为4.11%,其细胞亚群集落培养形成CFU-GM的集落数为(37±3)/1×103;形成CFU-Mix的集落数为(34±5)/1×103;形成CFU-E集落数为(33±4)/1×103。胎盘AC133-细胞未见集落形成。结论:人胎盘组织含HSPC,AC133+细胞具有分化为CFU-GM、CFU-Mix、CFU-E的能力;AC133膜抗原可作为胎盘HSPC的分选标志。To study the colony formation capability of AC133＋ cells in human placenta tissues. Single cells （nucleated cells） suspension was made from fresh human placenta tissue, the purity of AC133＋ hematopoietic stern/progenitor cells were analyzed by the flow cytometry （FCM）. The AC133＋ and AC133- HSPC cells were sorted by MACS and then cultured by the granulocyte/macrophage colony forming unit （CFU-GM）, colonyforming unit erythroid （CFU-E） and mixed forming unit （CFU-Mix） respectively for evaluating their proliferation and differentiation. In human PT, there was 4.11% AC133＋ cells, the number of colonies of CFU-GM, CFU-Mix and CFU-E were （37±3）/1＊103, （34±5）/1＊ 103 and （334±） 1 ＊ 103 respectively. No colony ofAC 133- HSPCs was found in the human placenta tissue. There were HSPCs in the human placenta tissue. The PT AC133＋ HSPCs cells can differentiate into CFU-GM, CFU-E, CFU- Mix. Thus suggesting that PT AC133＋ cells contain HSPCs and the AC133 membrane antigen could be the sign of sorting the HSPCs in PT.

关 键 词：人胎盘 AC133+细胞 造血干/祖细胞 集落培养 

分 类 号：R392.9[医药卫生—免疫学]