剑叶金鸡菊化学成分及生物活性研究  

Chemical constituents from Coreopsis lanceolata and their bioactivities

作　　者：邵东旭[1] 郑冬梅[1] 胡锐[1] 陈文[1] 陈多谋[1] 卓先勤[1] 

机构地区：[1]琼州学院理工学院,海南三亚572022

出　　处：《中草药》2013年第12期1558-1561,共4页Chinese Traditional and Herbal Drugs

基　　金：海南省教育厅高等学校科研资助项目(Hj2009-132)

摘　　要：目的首次对剑叶金鸡菊Coreopsis lanceolata全草的化学成分和抗菌活性进行研究。方法采用各种柱色谱进行分离纯化,通过波谱数据分析(MS、NMR等)进行结构鉴定。对化合物1～8的抗菌活性进行了初步筛选。结果从剑叶金鸡菊全草氯仿部位中共分离得到了15个化合物,其中包含8个倍半萜类化合物,分别鉴定为1β,5α-diangeloyloxy-eudesm-(15)-ene(1)、1β,6α-dihydroxyeudesm-4(15)-ene(2)、10α-hydroxyoplopan-4-one(3)、(7R*)-opposit-4(15)-ene-1β,7-diol(4)、(7R*)-opposit-4(15)-ene-1β,8-diol(5)、(7R*)-opposit-4(15)-ene-1β,11-diol(6)、(7R*)-opposit-4(15)-ene-1β,7α-diol(7)、4(15)-eudesmene-1β,7α-diol(8)、对羟基桂皮酸(9)、3-甲氧基-4-羟基苯甲酸(10)、对羟基苯甲酸甲酯(11)、3,4-二羟基苯甲醛(12)、β-谷甾醇(13)、木栓酮(14)、木栓醇(15)。结论所有化合物均为首次从该植物中分离得到,化合物3、4对金黄色葡萄球菌有较强的抑制作用。Objective To study the chemical constituents in the whole herb of Coreopsis lanceolata and to investigate the antibacterial activity.Methods The compounds in the whole herb of C.lanceolata were isolated and purified by column chromatography and identified based on spectral analyses（MS,NMR）.The antibacterial activities of compounds 1—8 were screened.Results Fifteen compounds including eight sesquiterpenes were isolated from the chloroform fraction in the whole herb of C.lanceolata and were identified as 1β,5α-diangeloyloxy-eudesm-（15）-ene（1）,1β,6α-dihydroxyeudesm-4（15）-ene（2）,10αhydroxyoplopan-4-one（3）,（7R＊）-opposit-4（15）-ene-1β,7-diol（4）,（7R＊）-opposit-4（15）-ene-1β,8-diol（5）,（7R＊）-opposit-4（15）ene-1β,11-diol（6）,（7R＊）-opposit-4（15）-ene-1β,7α-diol（7）,4（15）-eudesmene-1β,7α-diol（8）,p-hydroxy cinamic acid（9）,3-methoxy4-hydroxy-benzoic acid（10）,methyl-p-hydroxybenzoate（11）,3,4-dihydroxy-benzaldehyde（12）,β-sitosterol（13）,friedeline（14）,and friedelinol（15）.Conclusion Compounds 1—15 are obtained from the whole herb of C.lanceolata for the first time.The bioassays show that the compounds 3 and 4 have the stronger inhibition against Staphylococcus aureus.

关 键 词：剑叶金鸡菊 倍半萜 对羟基桂皮酸 3 4-二羟基苯甲醛 木栓酮 

分 类 号：R284.1[医药卫生—中药学]