液质联用技术鉴定预知子提取物中的主要化学成分  

Identification of main chemical constituents in extracts of Akebiae Fructus by HPLC-ESI-MS

作　　者：孟月华[1,2] 黄何松[1,2] 余黄鹏[2] 何明珍[1] 孙勇兵[1,2] 冯育林[1,2] 杨世林[1,2] 

机构地区：[1]江西中药固体制剂制造技术国家工程研究中心,江西南昌330006 [2]江西中医学院,江西南昌330006

出　　处：《中草药》2013年第12期1562-1567,共6页Chinese Traditional and Herbal Drugs

基　　金：国家"重大新药创制"科技重大专项(2012ZX09103201-008);江西省科技支撑计划项目(20111BBG70005-2);江西省自然科学基金(20122BAB205032;20114BAB215046)

摘　　要：目的建立分析鉴定预知子提取物中的主要化学成分的方法。方法采用LC-ESI-MS负离子检测模式对预知子中的皂苷类成分进行分析,对其进行多级质谱裂解分析。以对照品及文献数据为对照,通过对各成分的MS2和MS3谱图的解析对各成分进行指认。结果预知子中的17个皂苷类成分获得了良好的分离和鉴定。结论本方法准确快速,适合预知子中主要化学成分的鉴定,可用于预知子原药材的质量控制。Objective To establish an HPLC-ESI-MS method for quickly identifying the chemical constituents in the extracts of Akebiae Fructus.Methods The main saponin components in the extracts of Akebiae Fructus were detected with the HPLC-ESI-MS in negative ion mode.These components were further analyzed by MS2 and MS3 spectra,and by comparing with the corresponding reference substances and literature data.Results Seventeen saponins in the extracts of Akebiae Fructus were well separated in one run.Conclusion The new method is accurate and rapid.It could be used to identify the main chemical constituents in the extracts of Akebiae Fructus and be suitable for the quality control of Akebiae Fructus.

关 键 词：液质联用技术 预知子提取物 皂苷类 常春藤皂苷元 齐墩果酸 阿江榄仁酸 

分 类 号：R284.1[医药卫生—中药学]