不同醋制工艺对三棱有效成分群溶出的影响  

Effect of different vinegar processing technologies on dissolution of effective composition groups in Sparganii Rhizoma

作　　者：孙杰[1] 吴艺舟[2] 王芍 王静[1] 马博[1] 孙晓波[1] 

机构地区：[1]中国医学科学院北京协和医学院药用植物研究所,北京100193 [2]南京医科大学基础医学院,江苏南京210029 [3]西安市食品药品检验所,陕西西安710000

出　　处：《中草药》2013年第12期1593-1598,共6页Chinese Traditional and Herbal Drugs

基　　金：国家科技支撑计划项目(2008BAI51B02);国家科技部重大新药创制专项(2012ZX09501001-004;2012ZX09301002-001);中国博士后科学基金面上项目资助(2012M510360)

摘　　要：目的研究不同醋制工艺对三棱中的总黄酮、总生物碱、有效Al3+3类药效物质基础提取率及提取比率的影响。方法采用L9(34)正交试验设计,从醋酸质量浓度、醋制比例、醋浸时间、炒制温度4个因素方面,比较不同醋制工艺条件下,醋三棱的80%甲醇浸膏提取率,总黄酮、总生物碱、有效Al3+提取率与提取比率的差异。结果与三棱生药相比,使用少量乙酸的醋制工艺提高了醋三棱80%甲醇浸膏提取率,醋炒可以有效提高醋三棱的总黄酮、总生物碱与有效Al3+的提取率与提取比率,总黄酮的提取率与提取比率受炒制温度影响显著,而总生物碱的提取率与提取比率则受醋酸质量浓度的影响显著。含大量乙酸的醋制工艺可明显降低醋三棱80%甲醇浸膏提取率,但可提高80%甲醇浸膏中总生物碱与有效Al3+的提取比率。结论醋制工艺对醋三棱中不同药效物质群的提取率与提取比率有显著影响,将三棱应用于不同适应症时,适宜对症适当改变醋制工艺。Objective To study the dissolving yield and ratio fluctuation of Sparganii Rhizoma effective composition groups,including total flavonoids,total alkaloid,and active Al3＋,when processed with different vinegar processing technologies.Methods L9（34） orthogonal test design was used with acetic acid content（A）,vinegar ratio（B）,soaking time（C）,and frying temperature（D） as influencing factors.The differences of dissolving yield and ratio of 80% methanol extract,total flavonoids,total alkaloid,and active Al3＋ of processed Sparganii Rhizoma under different vinegar processing technologies were compared.Results Compared with crude Sparganii Rhizoma,the processed products with a little acetic acid had enhanced the dissolving yield of 80% methanol extract.Moreover,the processed Sparganii Rhizoma with vinegar had effectively enhanced the dissolving yield and ratio of total flavonoids,total alkaloid,and active Al3＋.The dissolving yield and ratio of total flavonoids were greatly impacted by frying temperature.Meanwhile,the alkaloid dissolving yield and ratio were most influenced by acetic acid content.The products processed with abundant acetic acid had obviously reduced the dissolving yield of 80% methanol extract,but had increased the alkaloid and active Al3＋ dissolving ratio in the extract.Conclusion The processed Sparganii Rhizoma with vinegar could obviously affect the dissolving yield and ratio of different pharmacodynamic compositions in Sparganii Rhizoma.Suitable processed products should be adopted when Sparganii Rhizoma is used in the different treatments.

关 键 词：三棱 醋制工艺 总黄酮 总生物碱 有效Al3+ 

分 类 号：R283.1[医药卫生—中药学]