KIF11结构域在真核细胞的表达及其对细胞增殖的影响  

Expression of KIF11 Domains and Their Effects on Cell Proliferation

作　　者：郑桂玉[1] 黄振强[1] 顾巍[1] 林立[1] 

机构地区：[1]汕头大学医学院病理生理学实验室,广东汕头515041

出　　处：《汕头大学医学院学报》2013年第2期85-88,F0002,共5页Journal of Shantou University Medical College

摘　　要：目的:研究不同KIF11蛋白结构域对细胞增殖的影响,并检测对人HEK293T细胞增殖的抑制效果。方法:PCR扩增全长KIF11编码的mRNA,将含有HA抗原表位的KIF11-motor、-tail等结构域克隆到真核表达载体中。观察这些结构域在HEK293T中的表达和对细胞增殖的影响。结果:表达载体转染后,KIF11-motor、-tail能在细胞内有效表达,且都能抑制细胞增殖。结论:KIF11-tail抑制细胞增殖的能力比KIF11-motor更有效。Objective： To investigate the effect of KIFll domains on cell proliferation, and to determine the inhibting effect of HEK293T cells proliferation. Methods： The entire mRNA coding for KIFll was cloned by PCR. HA epitope-tagged KIF11-motor and -tail domains were cloned into an expressing vector. The domains were transfected into HEK293T for examining their ability in inhibiting cell proliferation. Results： The KIFll-motor and -tail domains were successfully expressed and were able to repress cell proliferation. Conclusion： The ability of KIF11-tail domain to inhibit cell proliferation is more than that of KIF 11-motor.

关 键 词：KIF11蛋白结构域 基因克隆和表达 细胞增殖 

分 类 号：R34[医药卫生—基础医学]