Penetrating view of nano-structures in Aleochara verna spermatheca and flagellum by hard X-ray microscopy  

作　　者：张凯 李德娥 洪友丽 朱佩平 袁清习 黄万霞 高昆 周红章 吴自玉 

机构地区：[1]Institute of High Energy Physicsh,Chinese Academy of Sciences [2]Key Laboratory of Zoological Systematics and Evolution,Institute of Zoology,Chinese Academy of Sciences [3]National Synchrotron Radiation Laboratory,University of Science and Technology of China

出　　处：《Chinese Physics B》2013年第7期410-414,共5页中国物理B（英文版）

基　　金：the National Basic Research Program of China(Grant Nos.2012CB825800 and 2009CB930804);the Key Program of the National Natural Science Foundation of China(Grant Nos.10734070 and 10774144);the National Natural Science Foundation of China(Grant Nos.11205189,10979055,31071909,and 31272358);the Knowledge Innovation Program of the Chinese Academy of Sciences(Grant No.KJCX2-YW-N42)

摘　　要：A penetrating view of the three-dimensional nanostructure of female spermatheca and male flagellum in the species Aleochara verna is obtained with 100-nm resolution using a hard X-ray microscope, which provides a fast noninvasive imaging technology for insect morphology. Through introducing Zernike phase contrast and heavy metal staining, images taken at 8 keV displayed sufficient contrast for observing nanoscale fine structures, such as the spermatheca cochleate duct and the subapex of the flagellum, which have some implications for the study of the sperm transfer process and genital evolution in insects. This work shows that both the spatial resolution and the contrast characteristic of hard X-ray microscopy are quite promising for insect morphology studies and, particularly, provide an attractive alternative to the destructive techniques used for investigating internal soft tissues.A penetrating view of the three-dimensional nanostructure of female spermatheca and male flagellum in the species Aleochara verna is obtained with 100-nm resolution using a hard X-ray microscope, which provides a fast noninvasive imaging technology for insect morphology. Through introducing Zernike phase contrast and heavy metal staining, images taken at 8 keV displayed sufficient contrast for observing nanoscale fine structures, such as the spermatheca cochleate duct and the subapex of the flagellum, which have some implications for the study of the sperm transfer process and genital evolution in insects. This work shows that both the spatial resolution and the contrast characteristic of hard X-ray microscopy are quite promising for insect morphology studies and, particularly, provide an attractive alternative to the destructive techniques used for investigating internal soft tissues.

关 键 词：X-ray microscopy computed tomography synchrotron radiation source morphology 

分 类 号：Q6[生物学—生物物理学]