7种结核分枝杆菌重组蛋白的纯化及血清学特性  

The purify and serologic characteristics of 7 types of recombinant proteins from mycobacterium tuberculosis

作　　者：闫冀焕[1] 史玲莉[1] 韩伟[1] 崔鹰[1] 赵俊伟[2] 张舒林[2] 

机构地区：[1]河北国际旅行卫生保健中心,河北石家庄050051 [2]上海交通大学基础医学院

出　　处：《中国国境卫生检疫杂志》2013年第3期160-164,共5页Chinese Journal of Frontier Health and Quarantine

基　　金：河北省科技支撑计划项目(10276105D-39)

摘　　要：目的分离纯化结核分枝杆菌重组蛋白38-kDa、16-kDa、Mtb81、ESAT-6、CFP10、Rv3425、Rv3391,并比较7种蛋白的免疫学特性,评价其在结核血清学诊断中的应用价值。方法利用间接酶联免疫吸附试验评价确诊结核病人、非结核病人、健康人血清中7种重组蛋白的抗原性。结果成功分离纯化了7种重组蛋白;酶联免疫吸附试验结果表明,7种抗原均能有效区分确诊结核病人、非结核病人和健康人,其中阳性率最高的是ESAT-6为47.3%,特异性最高的是Rv3391为98.3%。联合抗原中阳性率和特异性最高的是CFP10+ESAT-6+Rv3391抗原组合。结论单一抗原中ESAT-6具有较高的免疫原性,联合抗原中CFP10+ESAT-6+Rv3391抗原具有很高的阳性率和特异性,在结核诊断具有很高的诊断潜力。Objective To isolate and purify 7 types of proteins （38-kDa, 16-kDa, Mtb81, ESAT-6, CFPIO, Rv3425, Rv3391） from mycobacterium tuberculosis, and to compare the immunologic characteristics of 7 types of proteins, to evaluate the value of 7 antigens from mycobacterium tuberculosis on the serodiagnosis of tuberculosis （TB）. Methods 7 types of proteins were used as antigen to screen the sera of tuberculosis （TB） patients （n=203）,healthy controls （n=58）and non-tuberculosis （NTB） patients （n=42） by the enzyme-linked immunosorbent assay （ELISA）. Results 7 types of proteins were isolated and purified from mycobacterium tuberculosis. There was significantly distinction between TB patients, healthy controls and non-tuberculosis by the 7 proteins. The highest sensitivities antibody detected by single antigen was ESAT-6（47.3%）, and the highest speeificities was Rv3391（98.3%）. The highest sensitivity and highest specificity of antibodies detected by multiple antigens was CFP10＋ESAT-6＋Rv3391. Conclusion As a single antigen of proteins from mycobacterium tuberculosis, the immunogenicity of ESAT-6 is better than the other 6 antigens. The multiple antigens of CFP10＋ ESAT-6＋ Rv3391 can increase the sensitivity and specificity of TB antibody detection, and it may be valuable in the diagnosis of TB.

关 键 词：结核分枝杆菌 重组蛋白 抗体 抗原 纯化 

分 类 号：R521[医药卫生—内科学]