炎性因子IL-1βIL-18对静脉移植血管再狭窄影响的动物实验研究  

Effects of the Inflammatory Cytokines Interleukin-1beta(IL-1β) and Interleukin-18(IL-18) in Restenosis of Vein Grafts after Vascular Transplantation

作　　者：李光 尤斌 李平 李玉玲 吴意娜 张聪聪 

机构地区：[1]北京市安贞医院心脏外科,北京100029 [2]北京市心肺研究所,北京100029

出　　处：《现代生物医学进展》2013年第18期3436-3440,3444,共6页Progress in Modern Biomedicine

基　　金：国家自然科学基金青年科学基金项目(81000090)

摘　　要：目的:通过观察炎性因子白细胞介素-1β、白细胞介素-18在小鼠静脉移植后再狭窄血管中的表达,为临床冠脉搭桥术后血管再狭窄的早期诊断和药物治疗提供潜在靶点。方法:48只雄性小鼠,其中24只小鼠取出下腔静脉作为供体,采用套管法移植至另外24只小鼠的右颈动脉。建立颈动静脉血管移植模型,成活后随机分成三组每组8只,分别在一周、四周、八周处死,取移植血管,观察移植血管的通畅情况、血管内膜、中膜的增殖情况及炎性因子IL-1β、IL-18的表达;以供体血管作为对照组。结果:24只模型小鼠均存活,移植静脉血管内膜、中膜不同程度增生,免疫组化结果显示,正常静脉无明显炎症细胞侵润,移植静脉在一周时组织中大量MAC-2阳性单核巨噬细胞侵润,细胞因子IL-1β、IL-18的表达与正常静脉相比明显增加,、IL-18的表达分别为(9.52±1.81)%VS(0.82±0.12)%;(7.51±1.31)%VS(0.69±0.06)%,均为P<0.05)。在四周、八周仍有大量巨噬细胞侵润,细胞因子IL-1β高表达,四周、八周IL-1β表达分别为(7.01±1.21)%、(2.48±0.62)%。移植静脉管壁逐渐增厚,管腔逐渐狭窄;与对照组静脉比较(56.15μm±4.65μm),一周、四周、八周血管内膜厚度显著增厚分别为(204.26μm±24.29μm 551.83μm±35.00μm 723.90μm±127.42μm,均为P<0.05)。结论:炎性因子IL-1β、IL-18在静脉移植血管再狭窄中的表达增加,参与静脉移植后血管再狭窄的发生,因此可通过抑制炎性因子IL-1β、IL-18来治疗冠脉搭桥后静脉再狭窄。Objective：This paper is to observe the expression of the inflammatory cytokines,specifically Interleukin-1beta（IL-1β） and Interleukin-18（IL-18） in restenosis of vein grafts after vascular transplantation in male mice.This study aimed to detect inflammatory marker molecules in vein graft by using an experimental mouse model and therefore provide potential targets in developing early diagnosis and drug therapy for restenosis of vein graft.Methods：48 male mice,24 mice to remove the inferior vena cava as donor and were killed.The inferior vena cavas transplanted to another 24 mice＇s the right carotid artery with the cuff method.The 24 model divided into three groups randomly,8 mice each group.And the mice were killed at 1,4,and 8 weeks.The vein grafts were collected to measure patency,hyperplasia of the intimal and medial,and the expression of the inflammatory cytokines IL-1β and IL-18,donor mice＇s blood vessels were used as the control group.Results：All 24 mice were survived.Vein graft intimal and medial showed varying degrees of hyperplasia.Immunohistochemical results show,but normal venous have not obvious inflammatory cell infiltration.Vein graft have been infiltrated with a large number of MAC-2-positive monocyte-macrophage cell,the expression of the inflammatory cytokines IL-1β and IL-18 were elevated（9.52±1.81）%VS（0.82±0.12）%,（0.31±0.91）%VS（0.69±0.06）%,P0.05.In the 4th week and the 8th week,there was still a large number of macrophages infiltrating high expression of cytokines,IL-1β expression were（7.01±1.21）%,（2.48±0.62）%.The transplant vein wall were gradually thickening,the lumen gradually narrow,compared with the control group vein（56.15 μm±4.65 μm）.At the first week,the 4th week and the 8th week,intimal thickness were markedly thickened（204.26μm±24.29 μm 551.83 μm±35.00 μm 723.90 μm±127.42 μm,P0.05）.Conclusion：Expression of the inflammatory cytokines IL-1β and IL-18 was elevated in vein grafts in mouse,which is suggested that the i

关 键 词：静脉移植血管 再狭窄 白细胞介素-1Β 白细胞介素-18 

分 类 号：Q95-3[生物学—动物学] R541.4[医药卫生—心血管疾病]