酒精性心肌病大鼠心肌解偶联蛋白2表达及能量代谢变化  

The Changes of Myocardial UCP2 Expression and Energy Metabolism in Alcoholic Cardiomyopathy Rats

作　　者：高艳辉[1] 陈延军[1] 刘广忠[2] 张玉卓[1] 吴建军[1] 韦宏[1] 贾静[1] 钟丽华[1] 

机构地区：[1]哈尔滨医科大学附属第四医院,黑龙江哈尔滨150001 [2]哈尔滨医科大学附属第一医院,黑龙江哈尔滨150001

出　　处：《现代生物医学进展》2013年第19期3637-3640,3699,共5页Progress in Modern Biomedicine

基　　金：黑龙江省教育厅科学技术研究项目(12521203)

摘　　要：目的:观察心肌解偶联蛋白2(uncoupling protein 2,UCP2)在酒精性心肌病(alcoholic cardiomyopathy,ACM)时表达变化及对心肌能量代谢的影响。方法:将Wistar大鼠分为三组,酒精组(A组,10只)、少量饮酒组(B组,7只)和对照组(C组,7只),三组给予相同饮食,酒精组通过采取逐渐增加饮用酒精浓度并长期定量摄入的方法建立ACM模型,少量饮酒组长期饮用少量低浓度酒精,对照组以水代酒。6个月后心脏彩超测定心功能;计算左室/体重指数;RT-PCR法测定心肌组织UCP2 mRNA表达;Westernblot法测定心肌UCP2蛋白表达;高效液相色谱分析法测定心肌三磷酸腺苷酸(ATP)、二磷酸腺苷酸(ADP)、单磷酸腺苷酸(AMP)和磷酸肌酸(PCr)含量。结果:酒精组左心室射血分数和左心室短轴缩短率均低于对照组和少量饮酒组(P均<0.01),左心室舒张末期内径则高于对照组和少量饮酒组(P<0.01),左室/体重指数明显增加(P<0.01);酒精组UCP2 mRNA及蛋白表达高于对照组和少量饮酒组(P均<0.01);酒精组ATP、ADP、AMP和PCr较对照组和少量饮酒组明显减少(P均<0.01),相关性分析显示心肌组织ATP水平与UCP2蛋白表达呈显著负相关(r=-0.896,P<0.01)。结论:ACM时心肌UCP2表达明显增加,导致心肌能量代谢障碍,恶化心脏功能。Objective： To observe the expression of myocardial uncoupling protein 2 （UCP2） changes in alcoholic cardiomyopathy （ACM） model rats. Methods： The rats were randomly divided into three groups： ACM group （group A, alcohol-fed）, control gronp（group C） and a little alcoholic drinking group （group B）.Six months later, myocardial function was evaluated by echocardiography. The ratio of left ventricular weight and body weight （LVW/BW） were calculated. The expression ofUCP2 mRNA in myocardium was detected by re- verse transcription-polymerse chain reaction （RT-PCR）; The UCP2 protein in myocardium was detected by western blot. The contents of ATP, ADP, AMP and PCr in myocardium were measured by high performance liquid chromatography （HPLC）. Results： Compared with control group and a little alcoholic drinking group, the cardiac function of ACM group aggravated and LVW/BW increased. Myocardial ATP, ADP, AMP and PCr contents also significantly decreased in ACM group than the other 2 groups. The mRNA and protein expres- sion of UCP2 were significantly increased in ACM group and negatively correlated with ATP contents. Conclusion： The expression of myocardial UCP2 was up-regulated in ACM rats and might be responsible for energy metabolism disturbance in this model.

关 键 词：酒精性心肌病 解偶联蛋白2 能量代谢 

分 类 号：Q953[生物学—动物学] R542.2[医药卫生—心血管疾病]