美伐他汀产生菌桔青霉表观遗传调控基因RpdA的克隆及生物信息学解析  

Molecular Cloning and Bioinformatics Analysis of the Epigenetic Regulation Gene RpdA in the Mevastatin-Producing Fungus Penicillium citrinum

作　　者：宋璨[1] 周峰[1] 胡昌华[1] 

机构地区：[1]西南大学药学院现代生物医药研究所,重庆400715

出　　处：《西南大学学报（自然科学版）》2013年第6期160-165,共6页Journal of Southwest University(Natural Science Edition)

基　　金：重庆市科技攻关重点项目(CSTC2012gg-yyjs10002-13)

摘　　要：为从表观遗传调控的角度对美伐他汀产生菌桔青霉进行分子操作和遗传育种,克隆桔青霉表观遗传调控基因组蛋白去乙酰化酶基因RpdA,对多种真菌的组蛋白去乙酰化酶编码基因序列比较分析,设计简并引物,以桔青霉cDNA和DNA为模板,结合3-race技术,克隆获取RpdA基因的全长序列,并对其蛋白结构进行生物信息学分析.研究发现:RpdA基因长2 072bp(GenBank登录号:KC417298),含4个外显子和3个内含子,cDNA开放阅读框长为1 092bp(GenBank登录号:KC417296),编码639个氨基酸,蛋白结构显示了较为保守的组蛋白去乙酰化酶ClassI家族结构特征.In order to clone and identify the histone deacetylase gene RpdA and provide candidate genes and molecular targets for epigenetic breeding in the mevastatin-producing fungus Penicillium citrinum, we ob- tained the full-length RpdA DNA sequence and cDNA sequence by means of the 3＇race technology and de- generate primers designed based on the known amino acid sequences of several Rpd3-type proteins. Analy- sis of this gene indicated that the full-length RpdA sequence was 2 072 bp （GenBank accession number： KC417298） with 4 exons and 3 introns and an open reading frame （ORF） 1 920 bp in length （GenBank ac- cession number： KC417296） which encodes a protein of 639 amino acid residues. The RpdA protein pres- ented a conserved sequence component which belongs to the Arginase-HDAC superfamily.

关 键 词：表现遗传 组蛋白去乙酰化酶 基因克隆 桔青霉 

分 类 号：Q933[生物学—微生物学]