小麦春化突变系T128的获得及变异分析  

Obtaining and Variation Analysis of Vernalization Mutant Line T128 of Wheat

作　　者：刘东军[1] 张宏纪[1] 郭长虹[2] 孙光祖[1] 孙岩[1] 郭怡璠[1] 刘文林[1] 杨淑萍[3] 闫文义[3] 王广金[3] 

机构地区：[1]黑龙江省农业科学院作物育种研究所,黑龙江哈尔滨150086 [2]哈尔滨师范大学分子细胞遗传与遗传育种黑龙江重点实验室,黑龙江哈尔滨150075 [3]黑龙江省农业科学院

出　　处：《黑龙江农业科学》2013年第7期1-4,共4页Heilongjiang Agricultural Sciences

基　　金：国家863课题资助项目(2012AA1012-1)

摘　　要：春小麦龙辐10号外源基因转化后代中发现了变异植株,并选育出弱冬习性的突变系T128。为了明确T128的突变机理,利用特异标记检测了龙辐10号和突变系T128的春化基因。结果表明:龙辐10号和T128的春化基因分别是Vrn-A1a、vrn-B1、vrn-D1、vrn-B3和vrn-A1、Vrn-B1、vrn-D1、vrn-B3。龙辐10号具有显性基因Vrn-A1a,而突变系T128的显性基因为Vrn-B1,致使突变系T128变为弱冬习性,生长发育时期延迟,表明外源基因转化过程可以导致基因变异。Agronomic trait variation was found in the offsprings of genetic transformation in wheat Longfu 10,and mutant line T128with medium winterness was bred.In order to determine mutation mechanism,vernalization genes of Longfu 10and mutant line T128were tested by designed mark.The result showed that vernalization genes type of Longfu10were Vrn-A1a,vrn-B1,vrn-D1and vrn-B3,that of mutant line T128were vrn-A1,Vrn-B1,vrn-D1and vrn-B3.Longfu 10had dominant gene Vrn-A1a,dominant gene of mutant line T128was Vrn-B1.It induced medium winterness of mutant line T128,delayed developmental stage.The research considered that genetic transformation induces variation of non-target agronomic traits.

关 键 词：小麦 春化突变系 春化基因 非目标性状变异 

分 类 号：S512.1[农业科学—作物学]