美国紫薯试管苗微型繁殖的研究  

Study on Micro Propagation of Tube Seedlings of the Ipomoea batatas

作　　者：曲艺姣[1] 周清[1] 孙佳[1] 徐娜[1] 姜长阳[1] 

机构地区：[1]辽宁师范大学生命科学学院,辽宁大连116081

出　　处：《黑龙江农业科学》2013年第7期10-12,共3页Heilongjiang Agricultural Sciences

基　　金：辽宁省普通高等教育本科教学改革研究资助项目(201203041-4);辽宁省大学生创新创业训练资助项目(201203015011)

摘　　要：为满足人们对经过热处理的美国紫薯试管苗种苗的大量需求,以微型嫩茎段为材料,采用组织培养和微型扦插的方法,进行了试管苗微型繁殖的研究。结果表明:在1/2MS+IAA0.2mg.L-1+蔗糖15g.L-1培养基上,以具有2个生长点和2个叶片的微型茎段繁殖试管苗,1株试管苗每年能繁殖200多万株试管苗;以具有2个生长点和2个叶片的苗微型茎段扦插到苗床上,其繁殖速度提高了1.8倍。定植的微型扦插试管苗生长较旺盛,美国紫薯的产量提高48.9%。In order to meet the large needs for tube seedlings of Ipomoea batatas after heat treatment,with the method of tissue culture and micro cutting,the mini tender stem segments were used as material to do the research on micro propagation of tube seedlings in vitro.The results demonstrated that in the media of 1 / 2MS＋ IAA 0.2mg.L^-1 ＋Sucrose 15g.L-1,one tube seedling,which reproduced by mini tender stem segments with 2 growing points and 2blades in vitro,could breed more than two million tube seedlings each year;with 2points and 2blades of micro cuttings to the seedbed propagation speed was increased by 1.8times.Colonization of the micro cutting plantlets grew strongly,the yield of Ipomoea batatas could increase by 48.9%.

关 键 词：美国紫薯 试管苗 微型扦插 

分 类 号：S531[农业科学—作物学]