4种裸胸鳝的分子遗传多样性和亲缘关系的RAPD分析  

RAPD analysis of molecular genetic diversity and genetic relationship of four Gymnothorax species

作　　者：杜民[1] 尹绍武[2] 刘艳红[1] 牛宝珍[1] 齐兴柱[3] 张本[3] 廖经球[3] 霍蕊[3] 

机构地区：[1]红河学院云南省高校农作物优质高效栽培与安全控制重点实验室,云南蒙自6611990 [2]南京师范大学生命科学学院,江苏南京210023 [3]海南大学海洋学院热带生物资源教育部重点实验室,海南海口570228

出　　处：《海洋通报》2013年第3期321-327,共7页Marine Science Bulletin

基　　金：国家"十一五"科技支撑计划重点项目(2007BAD29B03);江苏省高校优势学科建设工程资助项目

摘　　要：采用29个随机引物,对海南近海及西沙的野生群体黄边裸胸鳝(Gymnothorax flavimarginatus)、云纹裸胸鳝(Gym-nothorax chilospilus)、波纹裸胸鳝(Gymnothorax undulatus)及细斑裸胸鳝(Gymnothorax fimbriatus)的基因组DNA进行了RAPD分析,并用UPGMA法对这4种裸胸鳝进行聚类分析。研究结果表明:(1)黄边裸胸鳝、云纹裸胸鳝、波纹裸胸鳝及细斑裸胸鳝4种裸胸鳝群体内遗传多样性都较高,其多态位点比率分别为68.65%、57.00%、50.80%、67.42%,平均杂合度分别为0.222 1%、0.207 4%、0.166 6%、0.221 2%,表明南海野生裸胸鳝遗传变异水平较高,种质资源状况良好;(2)4种裸胸鳝种间遗传距离及聚类分析表明,黄边裸胸鳝与细斑裸胸鳝遗传距离最近(0.340 6);黄边裸胸鳝与云纹裸胸鳝的次之(0.386 8);云纹裸胸鳝与波纹裸胸鳝的最远(0.531 2)。(3)在7个引物中,有区分云纹裸胸鳝、波纹裸胸鳝及细斑裸胸鳝的特异性片段,可用于这些裸胸鳝的鉴定。The RAPD （Random Amplified Polymorphic DNA） technique was applied to study the genetic diversity and the genetic relationship of four Gymnothorax species, including four wild populations （ G. flavimarginatus, G. chilospilus, G. undulates and G. fimbriatus） captured from the offshore area of Hainan, China. 29 random primers were used in the, analysis, and the genetic relationship of these Gymnothorax species was analyzed by UPGMA. The results indicated as follows. First of all, the percentages of polymorphic loci of four populations of Gymnothorax, G. flavimarginatus, G. chilospilus, G. undulates and G. fimbriatus were 68.65 %, 57.00 %, 50.80 % and 67.42 %, respectively. The percentages of the heterozygosity of the above four populations of Gymnothorax were 0.222 1%, 0.207 4 %, 0.166 6 % and 0.221 2 %, respectively. It denoted that the level of genetic diversity of them was high and the genetic status was good. Secondly, the interspecies genetic distance and clustering analysis showed that： among four Gymnothorax species, the genetic distance between G. flavimarginatus and G. fimbriatus was minimum （0.340 6）,and that between G.flavimarginatus and G. chilospilus took the second place （0.386 8）, and that between G. chilospilus and G. undulates was maximum （0.531 2）. Finally, among the fragments generated by 8 primers, we found some specific fragments which can be used to identify these Gymnothorax.

关 键 词：裸胸鳝 分子遗传多样性 亲缘关系 RAPD分析 

分 类 号：Q953[生物学—动物学]