石杉碱甲对D-半乳糖致衰老大鼠海马及下丘Lamin A／C、prelamin A及FACE1表达的影响  

The effect of huperzine A on the levels of lamin A/C, prelamin A and FACE1 in the hippocampus and inferior col-licnlnsofD-galactose-treatedagingrat

作　　者：高占娟[1] 阮清伟[2] 胡晓娜[3] 刘方[3] 马海芬[3] 董方元[3] 保志军[3] 

机构地区：[1]复旦大学上海医学院,上海市200433 [2]复旦大学附属华东医院中心实验室,上海市老年医学研究所,上海市200040 [3]复旦大学附属华东医院消化内科,上海市200040

出　　处：《老年医学与保健》2013年第3期176-179,共4页Geriatrics & Health Care

摘　　要：目的研究D-半乳糖致衰老大鼠海马及下丘laminA／C、prelaminA、FACEl的水平变化，并进-步明确石杉碱甲对D-半乳糖诱导的这些蛋白变化的影响及其意义。方法36只健康雄性SD大鼠随机分成对照组、D-半乳糖治疗组和D-半乳糖联合石杉碱甲组。D-半乳糖组每天皮下注射D-半乳糖300mg／Kg，共8W；D-半乳糖联合石杉碱甲组每天D-半乳糖300mg／Kg联合石杉碱甲0．1mg／Kg共同皮下注射，共8W。对照组皮下注射等量生理盐水。应用蛋白免疫印迹杂交（Western blotting）方法检测3组大鼠海马、下丘laminA／C、prelaminA和FACEl的表达。结果海马中，laminA在对照组、D半乳糖＋石杉碱甲组、D-半乳糖组中逐渐降低，laminC水平逐渐升高，prelaminA在对照组中最高，D-半乳糖组与D-半乳糖＋石杉碱甲组无明显差异；FACEl在D-半乳糖＋石杉碱甲组水平最高，对照组中水平最低。下丘中，laminA水平在D-半乳糖＋石杉碱甲组最高，D-半乳糖组中最低；D-半乳糖组和D-半乳糖＋石杉碱甲组laminC水平较对照组升高，D-半乳糖组与D-半乳糖＋石杉碱甲组之间的差异无统计学意义；prelaminA水平在对照组中最低，D-半乳糖＋石杉碱甲组中最高；FACE1水平在对照组、D-半乳糖＋石杉碱甲组、D-半乳糖组中逐渐升高。结论LaminA降低、laminC及FACE1升高预示着D-半乳糖诱导的海马及下丘衰老。石杉碱甲可以在-定程度上改善部分指标的变化，说明它有-定的延缓海马及下丘衰老的作用。Objective To investigate the changes of lamin A/C, prelamin A, FACE1 in the hippocampus and inferior colliculus of D-galactose treated aging rats. And also, to identify the effects and significance of huperzine A on the proteins changes induced by D-galactose. Method Thirty-six healthy male SD rats were randomly assigned into three groups： normal group, D-galactose treatment group and D-galactose combined with huperzine A treatment group. D-galactose group were injected subcutaneously with D-galactose 300mg/Kg/d for eight weeks. D-galactose combined with huperzine A treatment group were injected subcutaneously with D-galactose 300mg/Kg/d and huperzine A 0. 1mg/Kg/d for eight weeks. The nor- mal group were injected with equal amount of normal saline every day. Then Western blotting was used to measure the ex-pression of lamin A/C, prelamin A and FACE1 in the hippocampus and inferior colliculus. Results In the hippocampus, lamin A were gradually decreased in normal, D-galactose combined with huperzine A and D-galactose treated groups. While, lamin C was increased in the three groups gradually. Prelamin A was highest in the normal group and there was no significant difference between D-galactose combined with huperzine A and D-galactose treated groups. FACE1 was highest in the D- galactose combined with huperzine A group and lowest in the nornal group. In the inferior colliculus, lamin A was highest in the D-galactose combined with huperzine A treated group and lowest in the D-galactose treated group. The levels of lamin C in D-galactose treated group and D-galactose combined with huperzine A treated group have no significant difference, but they were both higher than normal group. As for prelamin A, it was highest in the D-galactose combined with huperzine A group and lowest in the normal group. The levels of FACElin normal, D-galactose combined with huperzine A and D- galactose treated group were increased gradually. Conclusions Lower levels of lamin A~ higher levels of lamin C and FACE1 indicate hippocampus and

关 键 词：石杉碱甲 衰老 海马 下丘 LAMINS FACE1 

分 类 号：R338[医药卫生—人体生理学]