AKT在七氟醚后处理大鼠海马脑片缺氧无糖损伤中的作用  

The Protective Effect of AKT Signaling Pathway on Sevoflurane Postconditioning Against Oxygen-Glucose Deprivation Injury in Rat Hippocampal Slices

作　　者：李燕[1] 王胜[1] 代志刚[1] 

机构地区：[1]石河子大学医学院第一附属医院麻醉科,石河子832002

出　　处：《石河子大学学报（自然科学版）》2013年第3期345-348,共4页Journal of Shihezi University(Natural Science)

基　　金：石河子大学自然科学与技术创新项目(ZRKXYB-LH05)

摘　　要：为探讨AKT信号通路在七氟醚后处理大鼠海马脑片缺氧无糖(OGD)损伤中的作用,本研究选取体重250～300g雄性成年SD大鼠,麻醉后断头处死,剥离海马,制备海马脑片60张,将其随机分为5组:对照组(C组),OGD组,七氟醚后处理组(Sevo组),LY294002和七氟醚后处理组(LY+Sevo组),二甲基亚砜(DMSO)和七氟醚后处理组(DMSO+Sevo组)。采用电生理技术,细胞外记录CA1区群峰电位(PS)波幅;采用碘化丙啶染色法,测定细胞活力,分析脑片损伤的程度。结果表明:与C组比较,其余各组PS恢复程度和细胞活力降低(P<0.01)。与OGD组比较,Sevo组、DMSO+Sevo组PS恢复程度和细胞活力升高(P<0.01),LY+Sevo组PS恢复程度和细胞活力差异无统计学意义(P>0.05);与Sevo组比较,LY+Sevo组PS恢复程度和细胞活力降低(P<0.01),DMSO+Se-vo组PS恢复程度和细胞活力差异无统计学意义(P>0.05)。上述结果显示:七氟醚后处理可通过激活AKT信号通路减轻大鼠海马脑片缺氧无糖损伤。To evaluate the protective effect of AKT signaling pathway on sevoflurane postconditioning against oxygen-glucose deprivation（OGD）injury in rat hippocampal slices. Male adult SD rats weighing 250-300g were anesthetized with ether and decapitated. The hippocampi were removed and sagittally sliced（400/lm thick）and placed in artificial cerebral spinal fluid aerated with 95%O2～ 5%CO2. Sixty hippocampal slices were randomly divided into 5 groups（n=12 for each group） ：control group（group C）,OGD group, sevoflurane postconditioning group（group Sevo）, LY294002 ＋ sevoflurane postconditioning group（group LY＋ Sevo）, DMSO＋ sevoflurane postcon-ditioning group（group DMSO＋Sevo）. Electrophysiological technique was used to record the amplitude of population spike（PS）in the stratum pyramidale of CA1 region,and the degree of recovery of PS amplitude was calculated. The cell viability was determined by propidium iodide staining. Compared with group C, the degree of recovery of PS and cell viability were significantly decreased in the other groups（P〈0.01 ）. Compared with group OGD, the degree of recovery of PS and cell viability were significantly increased in groups Sevo, DMSO＋Sevo（P〈0.01 ）. While no significant change was found in group LY＋ Sevo（P〉0.05）. Compared with group Sevo, the de- gree of recovery of PS and cell viability were significantly decreased in group LY＋Sevo（P〈0.01）. No significant change was found in group DMSO--Sevo（P〉0.05）. Sevoflurane postconditioning can attenuate the OGD injury to rat hippocampal slices through activating AKT signaling pathway.

关 键 词：AKT丝氨酸 苏氨酸激酶 七氟醚 脑缺血 后处理 海马 

分 类 号：R96[医药卫生—药理学]