响应面法优化碱提斜生栅藻多糖的提取工艺  

Optimization of Alkali Extraction Conditions of Scnedesmus obliquus Polysaccharides by Response Surface Methodology

作　　者：贺莹莹[1] 徐小琳[1] 王思雨[1] 王长海[2] 代斌[1] 

机构地区：[1]石河子大学化学化工学院/新疆兵团化工绿色过程重点试验室/省部共建国家重点实验室培育基地,石河子832003 [2]南京农业大学资源与环境科学学院,南京210095

出　　处：《石河子大学学报（自然科学版）》2013年第3期371-376,共6页Journal of Shihezi University(Natural Science)

基　　金：国家科技支撑计划项目(2011BAD14B01)

摘　　要：微藻多糖具有抗氧化、抗病毒等多种生物学活性,但对微藻多糖的提取多采用热水浸提法,多糖提取率及提取效率较低。为了提高斜生栅藻多糖的提取率,通过单因素试验和Box-Benhnken中心组合实验设计,对影响斜生栅藻多糖提取率的主要因素碱液浓度、提取温度和提取时间进行了优化。实验结果表明,当碱液质量浓度为0.175mol/L、提取温度为80.8℃、提取时间为2.1h时,实验条件最优,斜生栅藻多糖的提取率可达到11.08%,与模型预测值接近;优化后的碱提斜生栅藻多糖提取率和提取效率较热水浸提法分别提高了4.74和13.27倍。Mieroalagal polysaccharides has been discovered to have higher anti-oxidation and antiviral activities,while the extraction technique of microalgal polysaccharides always use the hot water extraction which has lower extraction yield and efficiency. To improve the extraction yield of Scnedesmus obliquus polysaccharides, alkali concentration, extraction temperature,and extraction time affecting the extraction yield of Scnedesmus obliquus polysaccharides were optimized by using response surface methodology （RSM） on the basis of single-factor tests. The results showed that the optimum alkali concentration, extraction temperature, and extraction time of Scnedesmus obliquus polysaccharides extraction were 0. 175 mol · L-1, 80.8 ℃ and 2.1 h, respectively. Under these conditions, the experimental extraction yield of Scnedesmus obliquus polysaccharides was up to 11.08%, which matched well with the predicted one. The extraction yield and extraction efficiency of Scnedesmus obliquus polysaccharides with the optimized alkali extraction were higher than those with hot water extraction by 4. 74 and 13. 27 times,respectively.

关 键 词：斜生栅藻 多糖 提取 响应面优化 

分 类 号：TQ461[化学工程—制药化工] R284.2[医药卫生—中药学]