百色市献血人群中卡波氏肉瘤相关疱疹病毒DNA的套式PCR检测  

Detection of Kaposi′s sarcoma associated hepersvirus DNA in baise blood-donor crowd by nested PCR

作　　者：陈源红[1,2] 周正东 曾怡[2] 唐华英[2] 韦红玉[2] 樊晓晖[1] 

机构地区：[1]广西医科大学微生物学教研室,南宁530021 [2]右江民族医学院微生物学与免疫学教研室,广西百色533000 [3]广西百色市中心血站,533000

出　　处：《重庆医学》2013年第19期2204-2205,共2页Chongqing medicine

基　　金：国家自然科学资金资助项目(81071345;81160535);广西壮族自治区教育厅桂教科研(2012 201204LX316)

摘　　要：目的了解百色市献血人群中卡波氏肉瘤相关疱疹病毒(KSHV)的流行状况。方法收集百色市部分无偿献血者肘静脉抗凝血474份,其中男259份,女215份,采用套式聚合酶链反应(n-PCR)方法对474例样本所提取的DNA进行KSHV特异性序列扩增,1.5%琼脂糖凝胶电泳,出现168bp条带为阳性结果。结果 474名献血者中9例阳性,阳性率1.90%;其中男性献血者中检出阳性者5例,阳性率为1.93%;女性献血者中检出4例,阳性率为1.86%。男女两组间阳性率采用χ2检验,差异无统计学意义(P>0.05)。结论百色市献血人群中存在KSHV的感染,不同性别献血者感染率差异无显著性。Objective To understand the current epidemic situation of KSHV prevalence among blood donor crowd in Baise city.Methods There were 474 donors had been detected,including 259 male and 215 female.The KSHV DNA was detected by nested polymerase chain reaction（n-PCR）.Then there was a 168 bp strip for positive results in the 1.5% agarose gel electrophoresis.Results The DNA of KSHV was found in 9 of 474（1.90%）.The infection rate of male was 1.93%（5/259） and that of female was 1.86%（4/215）.The chi-square test analysis showed that the differences were not significant（P0.05） between the two groups of male and female.Conclusion The DNA of KSHV in Base blood-donor crowd was found,there was no significant difference of infection rate in different gender of blood-donor.

关 键 词：献血人群 卡波氏肉瘤相关疱疹病毒 套式聚合酶链反应 

分 类 号：R440[医药卫生—诊断学]