sTREM-1在铜绿假单胞菌感染和定植的表达及意义  

Expression and significance of sTREM-1 in pseudomonas aeruginosa infection and colonization

作　　者：高晓岚[1] 艾中平[1] 湛晓勤[1] 甘辞海[1] 

机构地区：[1]泸州医学院附属医院ICU,四川泸州646000

出　　处：《重庆医学》2013年第19期2246-2248,2251,共4页Chongqing medicine

基　　金：泸州医学院附属医院科研基金[院发(2011)43号]

摘　　要：目的通过研究可溶性髓样细胞触发受体-1(sTREM-1)在铜绿假单胞菌呼吸系统感染和定植不同状态下表达水平的变化,探讨sTREM-1在铜绿假单胞菌肺炎及呼吸道定植中的意义。方法将90只SD大鼠随机分为肺部感染组、口咽部定植组及对照组,每组按铜绿假单胞菌接种后不同时间又分为3、9、24h亚组,每亚组10只。运用酶联免疫吸附法(ELISA)检测血清sTREM-1浓度;采用逆转录-聚合酶链反应(RT-PCR)测定肺组织TREM-1mRNA表达;病理组织切片观察各组肺部炎症改变,并进行肺组织匀浆菌落计数。结果感染组大鼠肺组织病理改变在9h最为严重,而定植组和对照组的肺泡及肺间质结构基本正常;肺部感染组3、9、24h肺组织TREM-1mRNA的表达和血清中sTREM-1的浓度较定植组及对照组均明显增高(P<0.05),定植组与对照组TREM-1mRNA的表达和sTREM-1浓度的比较,差异无统计学意义(P>0.05)。结论检测sTREM-1有助于鉴别铜绿假单胞菌感染与定植;动态监测sTREM-1的变化对感染性疾病的早期诊断和病情评估具有指导意义。Objective To study the expression and significance of soluble triggering receptor expressed on myeloid cells-1（sTREM-1） in respiratory infection and colonization of Pseudomonas aeruginosa.Methods Ninety Sprague-Dawley（SD） rats were randomly divided into three groups：lung infection group,oropharynx colonization group and the control group.Each group was subdivided into 3,9,24 hours after being injected Pseudomonas aeruginosa,10 rats in each subgroup.The concentration of sTREM-1 was measured by enzyme-linked immunosorbent assay（ELISA）,the level of TREM-1 mRNA was measured by reverse transcriptase polymerase chain reaction（PCR）,lung pathological changes were observed,and the bacterial colonies of lung homogenate were counted.Results The changes of the lung tissue pathology were the most serious in the infection group at 9 hours.The alveolar and pulmonary interstitial structures were normal in the colonization and control groups.In the infection group of each time point,the expression of TREM-1 mRNA and the level of sTREM-1 were much higher than that in colonization group and control group（P0.05）,but there were no significant differences between the colonization and control group（P0.05）.Conclusion Detection of sTREM-1 will contribute to identify the infection and colonization induced by pseudomonas aeruginosa.Dynamic monitoring sTREM-1 on diagnosis and evaluation will have significance in infectious disease.

关 键 词：感染 铜绿假单胞菌 髓样细胞触发受体-1 定植 

分 类 号：R378[医药卫生—病原生物学]