双氢睾酮对人外周血内皮祖细胞增殖能力的影响  

Effect of dihydrotestosterone on proliferative ability of human endothelial progenitor cells*

作　　者：刘睿[1] 余明华[1] 曹政[2] 张鹏[1] 柯青[3] 黄铁柱[1] 

机构地区：[1]湖北医药学院基础医学院,湖北十堰442000 [2]湖北医药学院附属太和医院心内科,湖北十堰442000 [3]湖北医药学院附属太和医院肿瘤科,湖北十堰442000

出　　处：《重庆医学》2013年第20期2381-2383,共3页Chongqing medicine

基　　金：湖北医药学院2011年度研究生启动基金资助计划项目(2011QDZR-17);湖北省教育厅重点项目(D20092403);湖北省高校优秀中青年科技创新团队项目(T201008)

摘　　要：目的探讨双氢睾酮(DHT)对人外周血内皮祖细胞增殖能力的影响。方法采用密度梯度离心法从健康成年男性外周血获得单个核细胞,接种于人纤维连接蛋白包被的培养板。培养7d后,经Dil标记的乙酰化低密度脂蛋白(Dil-acLDL)及FITC标记的荆豆凝集素-I(FITC-UEA-I)荧光双染法鉴定正在分化的内皮祖细胞。采用人工计数法和3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐(MTT)法观察浓度为0nmol/L(对照组),1nmol/L DHT组,10nmol/L DHT组,100nmol/L DHT组DHT对内皮祖细胞增殖能力的影响。结果与对照组相比,各浓度DHT组内皮祖细胞增殖能力明显增强,差异有统计学意义(P<0.01);随着DHT浓度的升高促进作用逐渐增强,但浓度达100nmol/L时DHT对内皮祖细胞增殖能力促进作用有所减弱,但仍明显高于对照组,差异有统计学意义(P<0.01)。结论在一定浓度范围内,DHT可呈剂量依赖性增强内皮祖细胞增殖能力。Objective The purpose of this study is m explore the influence of dihydrotestosterone （I）HT） on human endothelial progenitor cells （EPCs） proliferation in vitro. Methods Peripheral blood mononuclear cells were isolaled from adult heahhy male blood donors. The cells were plated on fihronectin-coated plates and maintained in endothelial growth medium-2 （EC-M-2）. After 7 days, EPCs were identified by double-positive for DiI AeLDL and FITC UEA-I under laser scanning confocal microscope （LSCM）. In order to study the effect of DHT on EPCs,the cells were incubated witb a series of concentrations （0,1,10 andl00 nMol/L） of DHT for 2,1 h. The EPCs proliferation was detected by cell counting and 3-（4,5-dimethyhhiazol 2-yl）-2,5-diphenyl 2h tetrazolium bromide （MTT） assay. Results DHT significantly enhanced the proliferative ability of EPCs with DHT concentration increased （P〈〈0.01）. However, as the amount of DHT attained 100 nMol/I., proliferative ability of EPCs decreased to a level which was still significantly higher than tha＇, of control group. Conclusion DHT can enhance proliferation ability of EPUs with a dose dependent manner in certain limits in vitro.

关 键 词：前列腺肿瘤 双氢睾酮 内皮祖细胞 增殖能力 

分 类 号：R96[医药卫生—药理学]