高尔基体与细胞运动方向关系的体内外实验研究  

Relationship between Golgi apparatus and cell migration direction in vivo and in vitro

作　　者：刘彬[1] 刘志峰[1] 任炳成[1] 陈聪[1] 于圣平[1] 明浩朗[1] 王垒垒[1] 赵恺[1] 杨学军[1] 张斌[2] 

机构地区：[1]天津医科大学总医院神经外科天津市神经病学研究所神经肿瘤实验室,300052 [2]济宁医学院附属医院神经外科

出　　处：《中华医学杂志》2013年第25期2001-2003,共3页National Medical Journal of China

基　　金：国家自然科学基金（81272782）

摘　　要：目的 观察高尔基体与细胞运动方向的关系,探讨高尔基体作为细胞运动方向指示的可行性及其在研究细胞迁移运动方面的生物学意义.方法 选择大鼠C6胶质瘤细胞、人脑U251和SNB19胶质瘤细胞,通过细胞免疫荧光染色观察细胞迁移实验中高尔基体与细胞运动方向的关系,计数高尔基体朝向划痕空白区的细胞所占的百分比；鬼笔环肽细胞骨架染色,观察高尔基体与细胞运动伪足的位置关系；免疫组化染色观大鼠脑胶质瘤组织标本和人脑胶质瘤组织标本中坏死区周围细胞高尔基体的定位与细胞运动方向的关系,计数高尔基体背离坏死区的细胞所占百分比.结果 细胞迁移实验中,位于划痕两侧的绝大多数C6细胞（83％±6％）、U251细胞（80％±7％）、SNB19细胞（82％±6％）的高尔基体朝向划痕空白侧；鬼笔环肽细胞骨架染色显示U251细胞和SNB19细胞中高尔基体的定位方向与细胞的伪足伸展方向一致.免疫组化染色显示,肿瘤组织标本中坏死区周围肿瘤细胞的高尔基体多数（鼠脑80％±7％；人脑82％±6％）背向坏死侧.结论 无论在体内还是在体外环境中,细胞高尔基体的定位方向对于判断细胞的迁移运动方向都具有参考意义,从而为在体研究肿瘤细胞侵袭的生物学行为和抗迁移治疗疗效的观察提供了有力的观测指标.Objective To explore the relationship between Golgi apparatus and the direction of tumor cell migration in vivo and in vitro.Methods Cell migration assays were conducted with rat C6 glioma cells,human U251 and SNB19 glioma cells respectively.Then immunofluorescence was used to detect the position of Golgi apparatus in migrating cells.The percentage of cells with Golgi apparatus facing towards wound edge was calculated.Cell pseudopodium was stained with TRITC-phalloidin and the relationship between Golgi apparatus and pseudopodium detected.Immunohistochemistry was used to reveal the Golgi apparatus in tumor tissue samples.And the percentage of cells with Golgi apparatus facing opposite to the necrotic zones was calculated.Results In cells located at wound edge,the Golgi apparatus was found facing towards the wound in the vast majority of cells （C6 83% ±6%,U251 80% ±7%,SNB19 82% ± 6%）.In U251 and SNB19 cells,the golgi apparatus was located in the same direction with cellular pseudopodium.Immunohistochemical staining showed that in cells located around the necrotic zone,the Golgi apparatus faced opposite to the necrotic zones in most cells （rat tissue samples 80% ± 7%,human tissue samples 82% ± 6%）.Conclusion The Golgi apparatus is closely correlated with cell migration and it may be considered as a direction indicator of cell migration.And it provides an important index for the study of tumor cell invasion both in vivo and in vitro.

关 键 词：神经胶质瘤 细胞运动 高尔基体 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]