Serological and molecular capsular typing, antibiotic susceptibility and multilocus sequence typing of Streptococcuspneumoniae isolates from invasive and non-invasive infections  

作　　者：ZHANG Yi-jie CHEN Yu-shen WANG Zhan-wei LI Yu-qian WANG Da-xuan SHANG Ying FU Rong-rong HU Ying-hui GENG Rong WEI Li-ping YANG Jing-ping LI Jia-shu YU Qin DU Juan GAO Zhan-cheng 

机构地区：[1]Department of Respiratory and Critical Care Medicine (Zhang YJ, Li YQ, Shang Y, Fu RR and Gao ZC), Department of Clinical Laboratory [2]Peking University People's Hospital, Beijing 100044, China Department of Respiratory Medicine, Fujian Provincial Hospital, Fujian Medical University, Fuzhou, Fujian 350001, China [3]Department of Respiratory Medicine Beijing Children's Hospital Affiliated to Capital Medical University, Beijing 100045, China [4]Department of Emergency MedicineBeijing Children's Hospital Affiliated to Capital Medical University, Beijing 100045, China [5]Department of Respiratory Medicine, Third Affiliated Hospital of Guangzhou Medical College, Guangzhou, Guangdong 510150, China [6]Department of Respiratory and Critical Care Medicine, Third Affiliated Hospital of the Inner Mongolia Medical College, Baotou, Inner Mongolia 014030, China [7]Department of Respiratory Medicine, Lianyungang First People's Hospital Affiliated to Xuzhou Medical College, Lianyungang, Jiangsu 222002, China [8]Department of Respiratory Medicine, First Hospital of Lan Zhou University, Lanzhou, Gansu 730000, China [9]Department of Respiratory Medicine, Affiliated Hospital of Guiyang Medical College, Guiyang, Guizhou 550004, China

出　　处：《Chinese Medical Journal》2013年第12期2296-2303,共8页中华医学杂志（英文版）

摘　　要：Background Streptococcus pneumoniae （S.pneumoniae） is a major causative agent of severe infections,including sepsis,pneumonia,meningitis,and otitis media,and has become a major public health concern.We report the pneumococcal serotype and sequence type （ST） distribution,and antimicrobial resistance of 39 S.pneumoniae strains from seven hospitals in China.Methods Blood/cerebrospinal fluid （CSF） and sputum isolates from patients were analyzed to determine S.pneumoniae serotypes by polymerase chain reaction （PCR） and the Neufeld Quellung reaction,the multilocus sequence types （MLST） by PCR and sequencing,and susceptibility to antimicrobial agents by the VITEK Gram Positive Susceptibility Card.Results A total of 39 isolates were collected including 21 blood/CSF and 18 sputum isolates.Conventional serotyping by the Quellung reaction required 749 reactions.In contrast,PCR based typing needed only 106 PCR reactions.The most frequent serotypes from the blood/CSF isolates were 14 （38.1%）,19A （14.3%）,23F （9.5%）,and 18C （9.5%）.In the sputum isolates the most frequent serotypes were 19F （33.3%）,23F （16.7%）,19A （11.1%）,and 3 （11.1%）.The incidence of penicillin resistance in the blood/CSF and sputum isolates was 66.7% and 55.6%,respectively.Statistical analysis showed that patients ≤5 years old had a higher resistance to penicillin when they compared with the patients ≥65 years old （P=0.011）.Serotypes 14,19A and 19F were significantly associated with penicillin resistance （P 〈0.001）.ST320,ST271,and ST876 isolates showed high resistant rates to several antibiotics including penicillin （P=0.006）.All of the isolates of serotype 19A were resistant to both penicillin and erythromycin,and they were all multi-drug resistant （MDR） isolates.Conclusions The specificity and sensitivity of multiplexPCR are good,and this method represents a substantial savings of time and money,and can be widely used in the laboratory and clinical practice.Data from this research shoBackground Streptococcus pneumoniae （S.pneumoniae） is a major causative agent of severe infections,including sepsis,pneumonia,meningitis,and otitis media,and has become a major public health concern.We report the pneumococcal serotype and sequence type （ST） distribution,and antimicrobial resistance of 39 S.pneumoniae strains from seven hospitals in China.Methods Blood/cerebrospinal fluid （CSF） and sputum isolates from patients were analyzed to determine S.pneumoniae serotypes by polymerase chain reaction （PCR） and the Neufeld Quellung reaction,the multilocus sequence types （MLST） by PCR and sequencing,and susceptibility to antimicrobial agents by the VITEK Gram Positive Susceptibility Card.Results A total of 39 isolates were collected including 21 blood/CSF and 18 sputum isolates.Conventional serotyping by the Quellung reaction required 749 reactions.In contrast,PCR based typing needed only 106 PCR reactions.The most frequent serotypes from the blood/CSF isolates were 14 （38.1%）,19A （14.3%）,23F （9.5%）,and 18C （9.5%）.In the sputum isolates the most frequent serotypes were 19F （33.3%）,23F （16.7%）,19A （11.1%）,and 3 （11.1%）.The incidence of penicillin resistance in the blood/CSF and sputum isolates was 66.7% and 55.6%,respectively.Statistical analysis showed that patients ≤5 years old had a higher resistance to penicillin when they compared with the patients ≥65 years old （P=0.011）.Serotypes 14,19A and 19F were significantly associated with penicillin resistance （P 〈0.001）.ST320,ST271,and ST876 isolates showed high resistant rates to several antibiotics including penicillin （P=0.006）.All of the isolates of serotype 19A were resistant to both penicillin and erythromycin,and they were all multi-drug resistant （MDR） isolates.Conclusions The specificity and sensitivity of multiplexPCR are good,and this method represents a substantial savings of time and money,and can be widely used in the laboratory and clinical practice.Data from this research sho

关 键 词：SEROTYPE multilocus sequence typing antibiotic resistance Streptococcus pneumonia 

分 类 号：S858.316.3[农业科学—临床兽医学] TP275[农业科学—兽医学]