骨髓间充质干细胞对高氧肺损伤大鼠的治疗作用  

Therapeutic Effect of Bone Marrow Mesenchymal Stem Cells in Rat Hyperoxic Lung Injury

作　　者：张红珊[1] 方建培[1] 苏浩彬[1] 吴燕云[1] 

机构地区：[1]中山大学孙逸仙纪念医院儿科,广东广州501020

出　　处：《中山大学学报（医学科学版）》2013年第3期351-357,共7页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：广东省自然科学基金博士启动项目(S2012040008063)

摘　　要：【目的】:观察骨髓间充质干细胞(MSC)对新生大鼠高氧肺损伤的治疗作用。【方法】贴壁法体外培养扩增大鼠骨髓MSC,利用携带GFP基因的慢病毒液对骨髓MSC进行转染;SD新生大鼠随机分为A、B、C、D、E五组,A,B两组高氧暴露7 d后经尾静脉分别注射(1×105/只和1×104/只)骨髓MSC,C组注射磷酸盐缓冲液(PBS),D、E两组为空气组,分别注射骨髓MSC和PBS,观察骨髓MSC在大鼠肺组织的分布情况、大鼠的体质量增长、肺系数、放射性肺泡计数(RAC)及肺组织的病理改变。【结果】A、B、D组肺组织中均可见GFP+细胞;MSCs干预3、7 d,A,B两组GFP+细胞占总细胞数的比例高于D组(31.3±2.6%vs.11.8±1.3%,A组vs.D组,22.7±1.3%vs 11.8±1.3%,B组vs.D组,P<0.05);A组治疗第3、7、14天GFP+细胞占总细胞数的比例高于B组,P<0.05;A、B两组治疗后体质量增长加快(66±9 g vs.48±7 g,A组vs.C组,P<0.05),肺系数降低(2.26±0.23%vs.2.9±0.4%,A组vs.C组,P<0.05),RAC值明显增加(9.2±0.7 vs.7.4±0.4,A组vs.C组,P<0.05),肺部炎性反应减轻;A组对高氧肺损伤的治疗作用优于B组,P<0.05。【结论】两种剂量的骨髓MSC均可以减轻高氧肺损伤,高剂量组对高氧肺损伤的治疗效果更明显。[Objective]To investigate the therapeutic effect of bone marrow mesenchymal stem cells （MSC） in hyperoxic lung injury of neonatal rats.[Methods] Bone marrow MSC cultures were prepared from bone marrow of SD rats by adherent culture,and were transfected by lentiviral vector carrying GFP gene.Newborn rat pups from four different litters were randomized to A,B,C,D,and E groups.Different dose of bone marrow MSC （1 × 105 per pup or 1 × 104 per pup） were injected via the tail vein respectively for A and B groups after 7 days oxygen exposure,and phosphate buffered saline （PBS） for C group.MSCs and PBS were injected respectively for D and E groups as normoxia groups.The engraftment of MSCs in neonatal rat lungs,weight gaining,lung coefficient,radical alveolar counts and lung histology were assessed.[Results] GFP positive cells were found in rats lungs of A,B,D groups; the ratio of GFP-positive cells in A and B groups were higher than that of D group （31.3±2.6% vs 11.8±1.3%,A group vs D group,22.7 ± 1.3% vs 11.8 ± 1.3%,B group vs D group,P 〈 0.05） ; the ratio of A group was also higher than that of B group,P 〈 0.05）.Bone marrow MSC exerted their therapeutic benefit of improving weight gaining （66 ± 9 g vs 48 ± 7 g,A group vs C group,P 〈 0.05）,radical alveolar counts （9.2 ± 0.7 vs 7.4 ± 0.4,A group vs C group,P 〈 0.05）,decreasing lung coefficient （2.26 ± 0.23% vs 2.9 ±0.4%,A group vs C group,P 〈 0.05） and suppressing lung inflammation of neonatal rats,and which were significant better in the high dose group of MSCs treatment than that of low dose group.[Conclusion] Both two doses of bone marrow MSC treatment could attenuate hyperoxia-induced lung injury in newborn rats,and the therapeutic effect of high dose group was more obviously.

关 键 词：间充质干细胞 高氧 肺损伤 

分 类 号：R722[医药卫生—儿科]