纳米载银抗菌剂与四针状氧化锌抗白色念珠菌的性能  

Properties of nanometer silver antibacterial agent and tetrapod-like zinc oxide antibacterial agent against Candida albicans

作　　者：肖月[1] 康梁[1] 吕庆[1] 于博文[1] 马英君[1] 

机构地区：[1]佳木斯大学附属口腔医院,黑龙江省佳木斯市154002

出　　处：《中国组织工程研究》2013年第25期4609-4615,共7页Chinese Journal of Tissue Engineering Research

摘　　要：背景:口腔修复材料室温固化甲基丙烯酸甲酯的表面结构疏松多孔,极易附着各种细菌、微生物导致患者义齿性口炎的发生,所以在甲基丙烯酸甲酯中加入抗菌剂成为国内外学者研究的热点。目的:比较加入纳米载银抗菌剂与四针状氧化锌抗菌剂后,室温固化甲基丙烯酸甲酯对白色念珠菌的抗菌活性。方法:采用对倍稀释法测定纳米载银抗菌剂与四针状氧化锌抗菌剂对白色念珠菌的最小杀菌浓度。将纳米磷酸锆载银抗菌粉体与四针状氧化锌抗菌剂分别以0%,1%,2%,3%的质量比加入到室温固化甲基丙烯酸甲酯粉剂中,检测各组试件对白色念珠菌的抗菌率。结果与结论:纳米载银抗菌剂对白色念珠菌的最小杀菌浓度为40g/L,四针状氧化锌抗菌剂对白色念珠菌的最小杀菌浓度为25g/L。未加入纳米载银抗菌剂或四针状氧化锌抗菌剂的甲基丙烯酸甲酯几乎无抗菌活性,加入两种抗菌剂后其抗菌活性明显增加,随着加入抗菌剂质量比的增加,抗菌活性逐渐增强;当抗菌剂质量比增加到3%时,加入纳米载银抗菌剂的甲基丙烯酸甲酯抗菌率为92.23%,加入四针状氧化锌抗菌剂的甲基丙烯酸甲酯抗菌率为98.23%。表明纳米载银抗菌剂与四针状氧化锌抗菌剂对白色念珠菌均有抗菌效果,且四针状氧化锌抗菌剂比纳米载银系抗菌剂抗菌效果好。BACKGROUND： Surface structure of room temperature curing methyl methacrylate is loose and porous, easy to be attached to a variety of bacteria, microbes, leading to the occurrence of denture stomatitis. Thus antibacterial agent added in methyl methacrylate has been a hot focus in the study. OBJECTIVE： To compare the antibacterial activity of room temperature curing methyl methacrylate against Candida albicans after addition of the nanometer silver antibacterial agent and tetrapod-like zinc oxideantibacterial agent. METHODS： Minimum bactericidal concentration of nanometer silver antibacterial agent and tetrapod-like zinc oxide antibacterial agents against Candida a/bicans was determined by dilution method. The nanometer silver antibacterial agent loaded nanometer zirconium phosphate or tetrapod-like zinc oxide antibacterial agent with 0% 1%, 2%, 3% mass fraction were added to the room temperature curing methyl methacrylate to detect the antibacterial property against Candida a/bicans. RESULTS AND CONCLUSION： The minimum bactericidal concentration of nanometer silver antibacterial agent was 40 g/L. The minimum bactericidal concentration of tetrapod-like zinc oxide agent against Candida albicana was 25 g/L. Methyl methacrylate without addition of nanometer silver antibacterial agent or tetrapod-like zinc oxide antibacterial agent did not have antibacterial activity, and the antibacterial activity significantly increased afte~ addition of the two antibacterial agents. With the increased mass fraction of nanometer silver antibacterial agent or tetrapod-like zinc oxide antibacterial agent, the antibacterial properties of room temperature curing methyl methacrylate increased gradually. At 3% mass fraction, antibacterial rate of methyl methacrylate with nanometer silver antibacterial agent was 92.23%, and the antibacterial rate of methyl methacrylate with the tetrapod-like zinc oxide antibacterial agent was 98.23%. These results indicated that the nanometer silver antibacterial agent and tetrapod-Uke zinc oxide a

关 键 词：生物材料 组织工程口腔材料 甲基丙烯酸甲酯 纳米载银 四针状 氧化锌 白色念珠菌 抗菌剂 最小杀菌浓度 

分 类 号：R318[医药卫生—生物医学工程]