去细胞肌肉生物支架与人脐带间充质干细胞的相容性  

Biocompatibility of human umbilical cord mesenchymal stem cells and acellular muscle bioscaffolds

作　　者：张涛[1] 文益民[1] 李含[2] 魏祥科[2] 

机构地区：[1]解放军兰州军区兰州总医院脊柱外科,甘肃省兰州市730050 [2]兰州大学第二临床医学院,甘肃省兰州市730000

出　　处：《中国组织工程研究》2013年第25期4616-4622,共7页Chinese Journal of Tissue Engineering Research

基　　金：全军医学科学技术研究"十一五"计划课题资助项目(06MA081)~~

摘　　要：背景:去细胞肌肉生物支架联合人脐带间充质干细胞移植将是治疗脊髓损伤的一项重要措施。但两者是否具有良好的相容性,人脐带间充质干细胞能否在去细胞肌肉生物支架中长期存活并均匀分布,尚未得到证实。目的:观察大鼠去细胞肌肉生物支架与人脐带间充质干细胞的相容性。方法:改良化学法制备大鼠去细胞肌肉生物支架,将第3代人脐带间充质干细胞Hoechest33342荧光标记后分为3组进行实验,细胞+支架组、细胞+支架大鼠体内组和单纯细胞组。分别应用苏木精-伊红、Masson染色方法观察去细胞肌肉生物支架的组织形态,以荧光倒置相差显微镜和扫描电镜观察人脐带间充质干细胞的吸附和生长情况。结果与结论:人脐带间充质干细胞与去细胞肌肉生物支架充分附着,生长增殖活跃,细胞在支架内分布均匀。细胞+支架体内组与细胞+支架组相比在移植后1-7d人脐带间充质干细胞数量差异无显著性意义(P>0.05),在移植14d细胞+支架体内组人脐带间充质干细胞数量大于细胞+支架组(P<0.05)。提示去细胞肌肉生物支架与人脐带间充质干细胞有较好的相容性,体内环境更有利于细胞增殖和两者融合。BACKGROUND： Acellular muscle bioscaffold comb ned w th human umbilical cord mesenchymat stem cell transplantation is an important method for the treatment of spinal cord injury. But the compatibility between acellular muscle bioscaffolds and human umbilical cord mesenchymal stem cells, andwhether the human umbilical cord mesenchymal stem cells can distribute evenly in the acallular muscle bioscaffolds have not been confirmed. OBJECTIVE： To observe the compatibility of acallular muscle bioscaffolds and human umbilical cord mesenchymal stem cells. METHODS： The rat acellular muscle bioscaffolds were prepared with improved chemical method, and the passage 3 human umbilical cord mesenchymal stem calls were divided into three groups after labeled with Hoechest33342： call＋scaffold group, cell＋scaffold in vivo group and call group. The morphology of the acallular muscle bioscaffolds was observed with hematoxylin-eosin staining and Masson staining, and the adsorption and growth conditions of human umbilical cord mesenchymal stem cells were observed under fluorescence inverted phase contrast microscope and scanning electron microscope. RESULTS AND CONCLUSION： The human umbilical cord mesenchymal stem cells were fully attached to and actively grew on the acellular muscle bioscaffold, and distributed evenly. There was no significant difference in the number of human umbilical cord mesenchymal stem calls between the cell＋scaffold in vivo group and ceil＋scaffold group at 1-7 days after transplantation （P 〉 0.05）. At the 14 days after transplantation, the number of human umbilical cord mesenchymal stem calls in the call＋scaffold invivo group was larger than that in the call＋scaffold group （P 〈 0105）. There is a good compatibility between human umbilical cord mesenchymal stem cells and acellular muscle bioscaffold, and the in vivo environment is more conducive to cell proliferation and integration of cells and scaffolds.

关 键 词：生物材料 材料生物相容性 生物支架 肌基膜管 荧光标记 相容性 组织工程 其他基金 

分 类 号：R318[医药卫生—生物医学工程]