比较含红芪与含黄芪的玉屏风散煎剂含药血清对老龄小鼠脾淋巴细胞凋亡的影响  

Comparative study of Radix Hedyseri and Radix Astragali alternative Yupingfeng San on apoptosis of spleen lymphocyte in aging mice

作　　者：张俊英[1] 张李峰[1] 卫东锋[2] 程晨[1] 程卫东[1,3] 鲍英存[1] 李雪嫣[1] 

机构地区：[1]兰州大学基础医学院,兰州730000 [2]中国中医科学院中医临床基础医学研究所,北京100700 [3]南方医科大学中医药学院,广州510515

出　　处：《中药药理与临床》2013年第2期13-17,共5页Pharmacology and Clinics of Chinese Materia Medica

基　　金：甘肃省中医药科研立项课题(GZK-2012-43);中国博士后科学基金特别资助(201003073)

摘　　要：目的:应用中药血清药理学方法,观察含红芪与含黄芪玉屏风散含药血清对老龄小鼠脾淋巴细胞凋亡的影响。方法:用相同剂量的红芪替换玉屏风散中的黄芪后,应用透射电镜、流式细胞仪、Hoechst33258荧光染色、DNA琼脂糖凝胶电泳和免疫细胞化学法观察两方对老龄小鼠脾淋巴细胞超微结构、周期、DNA ladder、凋亡及Fas、FasL蛋白表达的影响。结果:与青龄组比较,老龄小鼠中凋亡的脾淋巴细胞数量增加,主要表现为核固缩;细胞周期中在非凋亡细胞二倍体峰(G1)之前出现凋亡峰,凋亡率显著增加,出现典型的凋亡特征"DNA ladder",Fas、FasL蛋白表达增强。含红芪与含黄芪玉屏风(6g生药/kg)含药血清均可减少凋亡的脾淋巴细胞及核碎片数量,减小凋亡峰,使凋亡率明显降低,"DNA ladder"明显减少,细胞膜表面Fas、FasL蛋白表达明显减少,但红芪和黄芪组间比较无明显差异。结论:老龄小鼠脾淋巴细胞凋亡的发生率增加,含红芪与含黄芪玉屏风散含药血清均可改善老龄小鼠脾淋巴细胞体外培养下凋亡的发生。To study the effect of Hedysari radix and Radix Astragali alternative Yupingfeng San containing serum on on apeptosis of spleen lymphocyte in aging mice using serum pharmacology method. Methods：Using the same dose of Radix Hedysari to replace the Radix Astragali in Yuping fengsan, transmission electron microscopy, flow cytometry, Hocchst 33258 fluorescence staining, DNA agarose gel elec- trophoresis and immunocytochemistry were employed to observe the effect of Yuping fengsan on apoptosis and Fas, FasL protein expression of spleen lymphocytes in aging mice. Results：Compared with youth mice, there were more apoptosis spleen lymphocytes and karyopyknosis in aging mice. Cell cycle analysis showed that Atypical subdiploid peak was observed. The percentages of apoptosis cells were significantly in- creased in aging group. The DNA ladder patterns were shown by agarose gel electrophoresis for DNA from spleen lymphocytes in aging mice and the expression level of Fas, FasL were enhanced. The Yuping Fang San containing Radix Hedysari and Radix Astragali （6g crude drugs/ kg） Drug-containing serum with Yupingfeng using Radix Hedyseri and Radix Astragali treatment decreased the number of spleen lymphocytes apeptosis, formation of nucleus fragments, subdiploid peak, apoptosis rate, typical DNA ladder stripe and expression level of Fas, FasL pro- tein, respectively. Furthermore, there were no significant difference between the Yuping Feng San containing Radix Hedysari and Radix As- tragali. Conclusion：Incidence of apeptosis was increased for spleen lymphocytes cultured in vitro in aging mouse. Drug-containing serum with Yupingfeng using Radix Hedyseri and Radix Astragali both could lower the occurrence of apoptosis of spleen lymphocytes in vitro in aging

关 键 词：红芪 黄芪 玉屏风散 老龄小鼠 脾淋巴细胞 细胞凋亡 

分 类 号：R285[医药卫生—中药学]