玉屏风散口服液和复芪止汗颗粒对免疫抑制小鼠的影响  

The immunomodulatory effects of Yupingfengsan and oral liquid Fuqizhihan granule act on immunosuppressive mice

作　　者：李雪嫣[1] 张李峰[1,2] 程卫东[1] 桂曼曼[1] 鲍英存[1] 

机构地区：[1]兰州大学中西医结合研究所 [2]兰州大学基础医学院免疫学研究所,兰州730000

出　　处：《中药药理与临床》2013年第2期17-20,共4页Pharmacology and Clinics of Chinese Materia Medica

基　　金：兰州大学中央高校基本科研业务费转向资金资助(lzujbky-2013-172)

摘　　要：目的:比较研究复芪止汗颗粒和玉屏风散口服液对免疫抑制小鼠的免疫调节作用。方法:通过给小鼠腹腔注射环磷酰胺制备免疫抑制模型小鼠,分别以生理盐水、玉屏风散口服液高(12g/kg)、中(6g/kg)、低(3g/kg)剂量和复芪止汗颗粒高(54g/kg)、中(27g/kg)、低(13.5g/kg)剂量灌胃,测定其器官指数、IL-1β、腹腔巨噬细胞吞噬指数、自然细胞杀伤活性、血清溶血素水平、IL-4、CD19+、T淋巴细胞增殖指数、T淋巴细胞亚群及IFN-γ水平。结果:各剂量组均能不同程度的拮抗环磷酰胺所致的细胞免疫抑制作用,作用基本相似,且在恢复脾脏指数、血清IFN-γ水平和CD3+T淋巴细胞百分比方面,玉屏风散各剂量组明显优于复芪止汗各剂量组(P<0.05)。结论:复芪止汗颗粒在提高免疫抑制小鼠免疫功能方面的作用与玉屏风散口服液相似,但玉屏风散口服液在提高小鼠细胞免疫功能方面效果优于复芪止汗颗粒。To compare and study the immunomodulatory effects of Fuqizhihan granule（FQZH） and Yupingfengsan oral liquid（YPFS） act on immunosuppressive mice. Method： Make immunosuppression model mice through introperitoneal inject cyclophosphamide, gavage normal saline, the mice in the normal group were intragastrically administrated with the same volume of distilled water, in the Yupingfengsan high- dose group （YPFSH）with YPFS 12g/kg, in the Yupingfengsan middle -dose group（YPFSM） with YPFS 6g/kg, in the Yupingfeng- san low -dose group（YPFSL） with YPFS 3g/kg for 15days. and the same ways in FQZH all groups, doses are changed according to different group, Fuqizhihan high -dose group （FQZH H）with FQZH 54g/kg, the Yupingfengsan middle -dose group（ FQZH M） with FQZH 27g/kg, the Yupingfengsan low - dose group（ FQZH L） with FQZH 14g/kg To determine organ index, serum IL - 1β, the phagocytic index of perito- neal macrophage, the natural killer cell activity, the level of serum hemolysin, the content of IL -4 and CD19 ＋ , the proliferation index of T lymphocyte, the content of T lymphocyte subsets and the level of serum IFN - . Results： Every dose group of two compounds could improve innate and adaptive immunity of immunosuppressed mice , and their extent on improving is similar. All dose group of yupinfeng san is better than all Fuqizhihan granule group in restoring spleen index, serum IFN - γlevel and CD3 ＋ T lymphocytes percentage, and a statistically sig- uifieant differences （ P 〈 0.05）, compare with same level dose, both middle group are the best in improving mice immunity, and the effect of YPFSM is better than FQZHM. Conclusion： Fuqizhihan granule and Yupingfengsan oral liquid is similar in e mice immu- nity improvement, especially middle group, but Yupinfeng san is superior to Fuqizhihan keli in restoring cell immunity for immunosuppressive mice.

关 键 词：玉屏风散口服液 复芪止汗颗粒 免疫抑制 固有免疫 适应性免疫 

分 类 号：R285[医药卫生—中药学]