莲心碱对大鼠局灶性脑缺血/再灌注损伤的抗氧化作用  

Study on antioxidation of liensinine on focal cerebral ischemia-reperfusion injury in rats

作　　者：余万桂[1] 张恒文[1] 杨涛 张道明[1] 邓德明[1] 

机构地区：[1]长江大学医学院,荆州434000 [2]荆州市中医院,荆州434000

出　　处：《中药药理与临床》2013年第2期32-35,共4页Pharmacology and Clinics of Chinese Materia Medica

基　　金：湖北省卫生厅科研基金资助项目(NO:JXzb74);荆州市医疗卫生科技发展计划(NO:20111307)

摘　　要：目的:探讨莲心碱对大鼠局灶性脑缺血/再灌注损伤的抗氧化作用。方法:40只大鼠随机分为5组,对照组、模型组组、莲心碱低、中、高剂量(舌静脉注射莲心碱1.5mg/kg、3mg/kg、6mg/kg),每组8只。采用线栓法阻塞大鼠大脑中动脉2h/再灌注24h制备脑缺血再灌注模型,观察莲心碱对大鼠局灶性脑缺血2 h再灌注24 h后神经功能障碍,脑水肿及脑梗塞范围以及脑组织能量物质ATP、葡萄糖无氧酵解产物以及体内自由基清除剂SOD、GSH和脂质过氧化产物MDA等生化物质的影响。结果:与对照组神经功能评分(0.00±0.00)比较,模型组神经功能评分(3.69±0.85)显著增高;与模型组比较,莲心碱中剂量组神经功能评分为(2.05±1.23)显著降低,莲心碱高剂量组神经功能评分(1.96±1.21)显著降低(P<0.01)。与对照组脑缺血体积比较,模型组脑缺血体积(28.91±3.71)%显著增加;而莲心碱低剂量组、中剂量组、高剂量组脑缺血体积分别为(25.36±3.52)%、(10.33±2.87)%、(9.91±2.61)%。与模型组比较,莲心碱低剂量组脑缺血体积无明显缩小;莲心碱中、高剂量组脑缺血体积明显缩小。与对照组脑组织含水量(77.33±4.34)比较,模型组脑组织含水量为(86.55±5.45)%明显高于假手术组(P<0.01),而莲心碱低剂量组、中剂量组、高剂量组脑组织含水量分别为(83.17±4.68)%、(80.03±4.59)%、(79.12±4.45)%;与模型组相比,莲心碱中剂量组、高剂量组脑组织含水量明显减少。与对照组大鼠血清SOD活性(61.44±15.23)、GSH-Px活性(225.91±8.81)、MDA含量(5.3±0.80)比较,模型组大鼠血清SOD活性(28.38±14.98)、GSH-Px活性(176.87±8.12)均显著降低(P<0.01),MDA含量(8.2±0.90)显著升高(P<0.01);与模型组比较,莲心碱中剂量组大鼠血清中SOD活性(54.23±18.32)显著升高,莲心碱高剂量组大鼠血清中SOD活性(57.12±17.51)显著升高(P<0.01);莲心碱中、高剂量组大鼠血清中GSH-Px活性为(199.61±12.67)、(206.13±14.To study the antioxidative effect of lieusinine on focal cerebral ischemia-reperfusion injury in rats. Methods： 40 SD rats were divided into 5 groups ： The sham group, the model group, the high dose of liensinine （ 6mg/kg）, the moderate dose of liensinine （ 3mg/ kg） ,the low dose of liensinine（1.5mg/kg）. The right middle cerebral artery of the rat was occluded by inserting a thread through internal ca- rotid artery for 2h, and then reperfused for 24h. Liensinine was injected after ischemia. After 24h, Use the Zea Longa grade point standard to valuate the hurt degree of the nervous system, the TYC dies calculate volume ratio of the cerebral ischemia. The activities of super-oxide dismutase （SOD）, glutathione peroxidase （GSH-Px） and the contents of malonic dialdehyde （MDA） in serum were assayed respectively. The levels of ATP and LA in brain were tested. Results： Compared with the sham group, the nervous system hurt symptom （3.69 ± 0. 85 ） was significant increased in the ischemia group. Compared with the isehemia group, the nervous system hurt symptom in the low dose of liensi- nine was （2.83 ± 1.31 ）, had no marked effect. The nervous system hurt symptom in the moderate dose of liensinine was （2.05 ± 1.23 ）, the nervous system hurt symptom in the high dose of liensinine was （ 1.96 ± 1.21 ）, was significant decreased. Compared with the sham group, the cerebral ischemia volume （ 28.91 ±3.71 ）% was significant increased in the ischemia group. Compared with the ischemia group, The cerebral ischemia volume in the low dose of lieusinine was （ 25.36 ±3.52） %, had no marked effect. The cerebral iachemia volume in the moderate dose of liensinine was（ 10.33 ± 2.87 ）%, the cerebral ischemia volume in the high dose of liensinine was（ 9.91 ± 2.61 ）%, had significant decreased. Compared with the sham group,the cerebral water content was （ 86.55 ± 5.45） % ,was significant increased in the ischemia group. Compared with the ischemia group, the cerebral wat

关 键 词：莲心碱 脑缺血 再灌注 抗氧化作用 能量代谢 

分 类 号：R285[医药卫生—中药学]