芒柄花素对兔离体肠平滑肌运动的影响及机制研究  

Influences of formononetin on contraction of small intestine smooth muscle of rabbits in vitro and its mechanism

作　　者：高雅[1] 邵正威[1] 贾晓宁[1] 王巧云[2] 

机构地区：[1]滨州医学院口腔学院 [2]滨州医学院基础学院,烟台264000

出　　处：《中药药理与临床》2013年第2期41-44,共4页Pharmacology and Clinics of Chinese Materia Medica

基　　金：2009年山东省高等学校优秀青年教师国内访问学者项目(No.2009GN003);2011年滨州医学院大学生科技创新项目(No.BY2011DKCX12)

摘　　要：目的:观察芒柄花素(formononetin)对兔离体肠平滑肌肌张力的影响,并探讨其作用机制。方法:试验采用经典的离体小肠灌流技术,观察芒柄花素对肠平滑肌自主收缩活动以及乙酰胆碱(acetylcholine,Ach)、氯化钡(Bacl2)及组胺(Histamine)所致痉挛性收缩肠平滑肌的影响。结果:20～100μmol/L芒柄花素能剂量依赖性的抑制家兔离体小肠平滑肌自发性收缩,对乙酰胆碱,组胺和氯化钡所致的兔离体肠痉挛性收缩也具有剂量依赖性抑制作用,且与肠平滑肌自发性收缩比较差异有显著性。在无钙台式液中,芒柄花素可明显抑制Ach引起的肠平滑肌收缩,亦能使CaCl2量效曲线下移,对Bay K8644(0.5μmol/L)引起的肠平滑肌收缩有明显抑制作用。结论:芒柄花素明显抑制了家兔离体小肠平滑肌的收缩活动,其机制与抑制外钙内流及内钙释放有关。To observe the influences of Formononetin on the contraction of small intestine smooth muscle of rabbits in vitro and explore the eehanism. Methods： With the isothermal perfusion of small intestine in vitro, the relaxant effects and related mechanism of Formononetin were studied on the spontaneous contraction of small intestine and contraction induced by Ach, histamine and Bacl2. Results： Formononetin （20 - 100μmol/L） reduced the contractivity of small intestine smooth muscle antonomic and agonized by acetylcholine ,BaCl2 and histamine （P 〈0.01 ）. Formononetin were found to inhibit the contraction induced bv acetychline in the Ca2＋- free Tyrode＇s solution and the CaC12 cu- mulative dose-response curves with a lower maximal response. And it can also depress the contraction agonized by Bay K8644 in small intes- tine smooth muscle. Conclusions ：Formononetin inhibits the contraction of small intestine smooth muscle of rabbits in vitro. The mechanism may be related to inhibits extracellular Ca2 ＋ inflowing via cell membrane and intracellular Ca2 ＋ releasing via sarcoplasmic reticulum.

关 键 词：芒柄花素 小肠平滑肌 钙离子 

分 类 号：R285[医药卫生—中药学]