地耳草总黄酮对5/6肾切除大鼠肾功能及组织纤维化的影响  

Effects of Hypterium japonicum total flavonoids on chronic renal failure rats induced by 5/6 nepgredtomy

作　　者：王强[1] 米军 李兴平[1] 刘家玉[1] 白筱璐[1] 

机构地区：[1]四川省中医药科学院,成都610041 [2]成都南山药业有限公司,成都610041

出　　处：《中药药理与临床》2013年第2期61-64,共4页Pharmacology and Clinics of Chinese Materia Medica

摘　　要：目的:观察地耳草总黄酮对5/6肾切除慢性肾功能衰竭(CRF)大鼠的作用及可能机制。方法:采用5/6肾切除复制大鼠CRF模型。实验分为模型对照组、阳性对照1组(金水宝胶囊)、阳性对照2组(卡托普利片),地耳草提取物低、中、高剂量组,假手术组共7组。灌胃给予相应药物或0.5%CMC-Na溶液1ml/100g。分别于给药28天、57天和89天,各组随机抽取部分大鼠腹主动脉采血,血清尿素氮(Bun)、肌酐(Cr)、层粘连蛋白(laminin,LN);处死大鼠,取肾脏固定,对肾脏进行切片,HE、Masson染色完成病理组织学检查,定量分析肾组织纤维化程度,免疫组化法检测转化生长因子-β1(TGF-β1)在肾组织中的表达。结果:连续给药28、57、89天,地耳草提取物各剂量组对5/6肾切除慢性肾功能衰竭大鼠均有一定的治疗作用,能降低模型大鼠Cr、Bun水平;降低血清LN含量及肾组织TGF-β1表达,使残余肾脏的肾小球、肾小管、肾间质病变及纤维化程度明显减轻,以120mg/kg、180mg/kg剂量组作用明显。结论:地耳草能改善慢性肾功能衰竭大鼠肾功能,其作用可能与通过TGF-β1途径改善肾组织纤维化病变程度有关。Objective: To study the effects of Hypterium Japonicum total flavonoids on chronic renal failure(CRF) Rats.Methods: CRF rat mode1 was estblished by 5/6 nephrectomy.After the modeling,sixty adult SD rats were randomly divided into 7 groups:positive control group 1(Jingshuibao capsules,580mg/kg),positive control group 2(Captopril Tablets,15mg/kg),high-,middle-and low-dose Hypterium Japonicum total flavonoids groups,sham-operation group and the model group.After the treatment for 28,57,89 days,renal function and renal patho1ogic changes were observed.Results:Compared with the model group,the serum Cr,Bun and LN were decreased in the positive control groups and Hypterium Japonicum total flavonoids groups.The expression of TGF-β1 and renal pathologic changes were signifieantly relieved than that of the model grou,especially in 120mg/kg,180mg/kg dose groups.Conlusion:Under the conditions of this experiment,Hypterium Japonicum total flavonoids can improve renal function in chronic renal failure rats,the effects may be related to the TGF-β 1 ways to improve the degree of renal fibrosis.

关 键 词：地耳草总黄酮 肾功能衰竭 纤维化 

分 类 号：R285[医药卫生—中药学]