大黄、巴豆霜对溃疡性结肠炎大鼠CD4~＋CD25~＋Treg的影响  

Mechanisms of Semen Crotonis Pulveratum and Rhubarb intervening CD4+CD25+/CD4+Treg in rats with ulcerative colitis

作　　者：王晓红[1] 侯丽娟[2] 孙晓萍[2] 唐方[1] 

机构地区：[1]天津医科大学总医院中医科 [2]天津医科大学,天津300052

出　　处：《中药药理与临床》2013年第2期127-129,共3页Pharmacology and Clinics of Chinese Materia Medica

摘　　要：目的:探讨巴豆霜、大黄及其成分是否通过维持CD4+CD25+Treg平衡,发挥溃疡性结肠炎(UC)大鼠的治疗作用。方法:采用2,4,6-三硝基苯磺酸(TNBS)法复制UC大鼠模型,将40只大鼠随机分为空白对照组、模型组、巴豆霜+大黄组、巴豆霜+没食子酸组、巴豆霜+大黄素组。观察结肠大体形态损伤、组织学变化,测定各组大鼠外周血CD4+CD25+Treg、CD4+CD25+Foxp3+Treg淋巴细胞变化。结果:与正常组比较,模型组大鼠外周血Treg细胞降低显著(P<0.01)。与模型组相比,巴豆霜+大黄组、巴豆霜+没食子酸组结肠大体形态损伤、组织学变化显著改善。治疗1周后,和模型组比较,巴豆霜+大黄组,巴豆霜+没食子酸组大鼠外周血Treg细胞有显著增高(P<0.01或P<0.05),且两组间比较没有差异;而巴豆霜+大黄素组没有差异(P>0.05)。结论:巴豆霜+大黄、巴豆霜+没食子酸对大鼠UC具有显著治疗作用,提高大鼠外周血CD4+CD25+Treg、CD4+CD25+Foxp3+Treg细胞可能是其作用机制之一,没食子酸是大黄和巴豆霜配伍发挥止泻作用的有效物质之一。To explore the therapeutic effect of Semen Crotonis Pulveratum, Rhubarb and it＇s component by keeping CIM ＋ CD25/CIM ＋ Treg, CIM ＋ CIY25 ＋ Foxp3/CD4 ＋ Treg balance in Wister rats with ulcerative colitis （UC）. Methods： Ratsi were randomized into 5 groups： normal control group, the model group, Semen Crotonis Pulveratum ＋ Rhubarb,Semen Crotonis Pulveratum ＋ Emodin, Semen Crotonis Pul- veratum ＋ Gallic acidgroup with 10 rats in each group and UC was induced by administrating 2, 4, 6tfinitrobenze-sulphonic acid （TNBS） in rats. Rats were sacrificed to observe the general morphology and histopathological changes of the colon, and detected the percentages of CD4 ＋ CD25 ＋ Tceg,CIM＋ CIY25 ＋ Foxp3 ＋ Treg in peripheral blood. Results： Compare to the model group, abnormally macroscopical, histological changes were improved in BDS ＋ DH,and BDS ＋ MSZ groupso The percentages of CIM ＋ CD25 ＋ Foxp3/CIM＋ Treg in peripheral blood in the model group is significantly lower than those in normal control group （ P 〈 0.01 ） ,while significantly high After treating one week in BDS ＋ DH, BDS ＋ MSZ groups （ P 〈 0.01 or P 〈 0.05 ）. No improvement or change was found in group BDS ＋ DHS. Conclusion： BDS ＋ DH, BDS ＋ MSZ have positive effects in treating colitis in rats by increasing the percentages of CD4 ＋ CD25 ＋ Foxp3/CIM ＋ Treg in peripheral blood.

关 键 词：巴豆霜、大黄 溃疡性结肠炎 TREG 

分 类 号：R285[医药卫生—中药学]