TRPV1通道蛋白介导的芍药甘草汤止痛配伍机制研究  

Study on the analgesic and formulation mechanisms of Shaoyao Gancao decoction

作　　者：李从越[1] 隋峰[2] 霍海如[2] 李兰芳[2] 郭淑英[2] 张春凤[3] 王君[3] 李沧海[2] 姜廷良[2] 

机构地区：[1]安徽中医学院,合肥230038 [2]中国中医科学院中药研究所,北京100700 [3]中国药科大学,南京211198

出　　处：《中药药理与临床》2013年第3期2-5,共4页Pharmacology and Clinics of Chinese Materia Medica

基　　金：国家自然基金(No.30873393;81274112);北京市自然基金(No.7092074);中国中医科学院“名医名家传承”项目(No.CM20123002)

摘　　要：目的:探讨芍药甘草汤止痛的分子作用机制和止痛的配伍机理。方法:大鼠造模前后及不同配伍比例的芍药甘草汤给药后的行为学、体重以及痛阈的变化,并采用荧光定量PCR(Real-time PCR)和免疫组化法观察TRPV1基因和蛋白表达的变化。结果:模型组大鼠体重相对较轻,大鼠后足造模侧足温升高、足趾肿胀、痛阈降低,造模对侧足在炎症继发期也出现类似症状,模型大鼠TRPV1通道基因和蛋白的表达升高;不同配伍比例的芍药甘草汤给药后均能缓解造模组大鼠的症状,并能够显著下调模型组大鼠TRPV1通道基因和蛋白的表达,但以芍药和甘草1:1配伍效果最为显著。结论:下调TRPV1通道蛋白的表达是芍药甘草汤抗炎止痛的分子作用机制之一;在止痛的效应环节,芍药和甘草1:1配伍比例最优。To investigate the molecular mechanisms of action and compatibility mechanisms of peony and licorice decoction. Methods： The gene and protein expression changes of TRPV1 was determined by using fluorescence quantitative PCR （ Real-time PCR） and immunohistechemical methods. Results： The formulas with different proportion of the peony and licorice decoction could downregulate the gene and protein expression of TRPV1 channel in the rats with adjuvant arthritis greatly （ P 〈0.05 or P 〈 0.01 ）, but the formula with equal amounts of peony and licorice is the most significant. Conclusion： Intervention of the expression level of TRPV1 channel is one of the molecular mechanisms of analgesic action for shaoyao Gancao decoction and application in the proportion of 1 to 1 of peony and licorice is the formula to a- chieve the maximum effects.

关 键 词：芍药甘草汤 TRPV1通道 止痛 佐剂性关节炎 

分 类 号：R285[医药卫生—中药学]