山药多糖对神经细胞毒性及抗缺氧/复氧诱导的神经细胞凋亡的影响  

Study of Chinese Yam polysaccharide on anti-apoptosis induced by hypoxia/reoxygenation

作　　者：向勤[1] 胡微煦[2] 蒲明[3] 文珠 何丹[3] 朱喜玲[1] 胡国柱[3] 

机构地区：[1]南昌大学研究生院医学部,南昌330006 [2]复旦大学上海市肿瘤医院,上海200032 [3]江西省人民医院,南昌330006 [4]江西省医学科学研究院,南昌330006

出　　处：《中药药理与临床》2013年第3期94-97,共4页Pharmacology and Clinics of Chinese Materia Medica

基　　金：江西省科技厅社会发展计划(2007BS22602)

摘　　要：目的:探讨山药多糖抗神经细胞缺氧性凋亡作用。方法:研究分为正常对照组、凋亡诱导组、山药多糖0.025g/L组、山药多糖0.05g/L组、山药多糖0.1g/L组、山药多糖0.25g/L组。采用"Neurobasal+B27"体外神经细胞无血清培养,免疫细胞化学鉴定神经细胞,MTT测定药物毒性,AnnexinV/PI双染流式细胞仪及Hochest33342荧光染色检测细胞凋亡。结果:山药多糖在2.0g/L之内预处理神经细胞对其无毒性作用,山药多糖在0.05g/L～0.1g/L范围显著地改善缺氧对神经细胞生长的抑制,0.025g/L～0.25g/L范围内显著地抑制缺氧复氧诱导的神经细胞早期凋亡。结论:山药多糖在一定浓度范围内具有抗缺氧复氧诱导的细胞早期凋亡作用。Objective：To explore the effect of Chinese Yam polysaccharide on preventing the neuron apoptosis indued by hypoxia.Methods： The study was divided into normal control group,apoptosis positive group induced by hypoxia,experiment 1 group treated by 0.025g/L Chinese Yam polysaccharide,experiment 2 group treated by 0.05g/L Chinese Yam polysaccharide group,experiment 3 group treated by 0.1g/L Chinese Yam polysaccharide group,experiment 4 group treated by 0.25 g/L Chinese Yam polysaccharide group.Neural cells were cultured by Neurobasal ＋ B27 in serum-free in vitro.Neural cells were identified by immunocytochemistry.Drug toxicity was determined by MTT assay,Neuron apoptosis was examined by Annexin V-FITC/PI in flow cytometry and Hoechst 33342 fluorescent staining.Results： The percentage of neural cells was 93.70% ± 0.56% in serum-free culture.The Chinese Yam polysaccharides was non-toxic for neural cells pretreated within 2.0 g/L.The Chinese Yam polysaccharides significantly prevented the inhibition of neural cell growth from hypoxia from 0.05g/L to 0.1g/L,（P 0.05）.Early apoptosis of neural cells induced by hypoxia-reoxygenation was significantly inhibited by Chinese Yam polysaccharides from 0.025g/L to 0.25g/L（P 0.05）.Conclusion： The Chinese Yam polysaccharide has an effect on inhibitting early apoptosis of neural cells induced by hypoxia-reoxygenation in a certain concentration range.

关 键 词：山药多糖 神经细胞 毒性 缺氧 凋亡 

分 类 号：R285[医药卫生—中药学]