北沙参与藜芦配伍对大鼠体内细胞色素P450酶活性的影响  

Compatible effects of Radix Glehniae and Veratrum nigrum on cytochrome P450 enzymes activities in rat livers

作　　者：朱冠秀[1] 王宇光[1] 马增春[1] 梁乾德[1] 肖成荣[1] 谭洪玲[1] 汤响林[1] 高月[1] 

机构地区：[1]军事医学科学院放射与辐射医学研究所,北京100850

出　　处：《中药药理与临床》2013年第3期107-111,共5页Pharmacology and Clinics of Chinese Materia Medica

基　　金：国家重点基础研究发展计划(No.2011CB505304;No.2012CB518402)资助项目

摘　　要：目的:研究中药十八反:北沙参与藜芦配伍对P450同工酶的影响,以阐明二者配伍基于药物代谢酶的层面产生相反作用的机制。方法:采用Cocktail底物探针法结合HPLC同时测定大鼠体内CYP1A2、CYP3A4、CYP2C9及CYP2C19的酶活性。结果:与空白组相比,黎芦组(0.81g生药/kg)、北沙参组(10.8g生药/kg)对大鼠CYP2C9酶具有诱导作用,合用组(11.61g生药/kg)对大鼠CYP1A2、CYP2C9酶具有显著诱导作用;与藜芦组相比,合用组对大鼠CYP3A4酶具有抑制作用,对CYP1A2、CYP2C9酶具有诱导作用;与北沙参组相比,合用组对大鼠CYP3A4酶具有抑制作用,对CYP1A2酶具有诱导作用,对CYP2C9、CYP2C19无明显影响。结论:北沙参与藜芦配伍前后对部分CYP亚型的酶活性影响具有差异,可能会使藜芦中的毒性成分在体内的代谢特征发生改变,使配伍前后毒性具有差异,产生中药间相互作用,但需进一步通过代谢研究并综合分析,以进一步阐明相反作用的机制。Objective：To study the compatible effects of Radix Glehniae and Veratrum nigrum on cytochromeP450 isoenzymes activities in rat livers and elucidate the imcompatible mechanism of traditional Chinese Medicine.Methods： The influence of Radix Glehniae coadministrated with Veratrum nigrum on cytochromeP450 isoforms CYP1A2,CYP3A4,CYP2C9 and CYP2C19 were simultaneous determined by cocktail probe drugs in rats.Results： Compared with the blank control group,Radix Glehniae（0.81g crude drug/kg） and Veratrum nigrum（10.8g crude drug/kg） can induce CYP2C9 activities,combined groups（11.61g crude drug/kg） acan induce CYP1A2 and CYP2C9 activities significantly;Compared with the Veratrum nigrum group,combined can inhibit CYP3A4 activity and induce CYP1A2 and CYP2C9 activity significantly;Compared with the Radix Glehniae group,CYP3A4 activities was significantly inhibited and CYP1A2 activities was significantly induced in combined groups,and had no effects on the activities of CYP2C9 and CYP2C19.Conclusion： These results indicated that compatibilit y of Radix Glehniae and Veratrum nigrum in a prescription have effect on the activity of cytochrome and its subtype enzymes,which may influence the toxic component＇s metabolism,phamacological effect and the toxicity,resultedin herb-herb interactions based on P450.The possible mechanisms of its incompatible effects need to be further analysised via integrating with metabolic studies.

关 键 词：北沙参 藜芦 十八反 细胞色素450 COCKTAIL 

分 类 号：R285[医药卫生—中药学]