三磷酸腺苷生物荧光法监测宫腔吸管清洗质量的研究’  

Study on ATP bioluminescence in assaying the cleaning quality of uterine suction

作　　者：常香远[1] 汪道新[2] 

机构地区：[1]深圳市第二人民医院消毒供应中心,广东省深圳518035 [2]深圳市第二人民医院烧伤科,广东省深圳518035

出　　处：《中国基层医药》2013年第14期2086-2088,共3页Chinese Journal of Primary Medicine and Pharmacy

基　　金：广东省深圳市科技计划项目（201102045）

摘　　要：目的探讨三磷酸腺苷（ATP）生物荧光法在监测官腔吸管清洗质量中的应用价值j方法按随机原则选择使用后的宫腔吸管120件，A组60件采用纯水浸泡＋高压水枪冲洗＋干燥的方法，B组60件采用碱性清洗液浸泡＋超声＋高压水枪冲洗＋手工擦洗＋干燥的方法。用目测法初步检测宫腔吸管清洗后的质量，用ATP生物荧光法和细菌计数法作平行比较，检测官腔吸管头内部顶端、吸管接头端内口2．5em处、吸管外壁前端、吸管腔内壁10am处，清洗前后细菌的残留量。结果两组宫腔吸管完成清洗干燥程序后吸管头内部顶端、吸管接头端内口2．5cm处、吸管外壁前端、吸管腔内壁10cm处相对光单位值（RLU）差异均有统计学意义（t=26．81、29．13、7．14、55．74，均P〈0．01），B组清洗效果明显优于A组。细菌计数法检测未检出致病微生物，上述四个部位清洗合格率分别是：A组：70．0％、85．0％、93．3％、93．3％；B组：95．0％、98．3％、100．0％、100．O％；B组合格率均高于A组（X^2=12．99、6．98、4．14、4．14，均P〈0．05）。结论碱性清洗液浸泡、超声和手工擦洗是官腔吸管清洗的重要环节；ATP生物荧光法可动态地快速检测官腔吸管清洗前后的细菌残留量，能作为有效检测官腔吸管清洗质量的评价方法。Objective To explore the application value of ATP bioluminescence in assaying the cleaning quality of uterine suction. Methods According to the principle of random, we selected 120 uterine suction tube after use. 60 used tubes in A group were given water immersion ＋ high-pressure water jets wash ＋ dry method ,60 used tubes in B group were given alkaline cleaning solution soak ＋ ultrasonic ＋ high-pressure water washing ＋ manual scrub ＋ drying method. The quality of suction tube was initially tested with the visual method after cleaning, Straw inside the top cavity,2.5em at inside straw joint and the front of straw outer wall, 10era at suction cavity wall, before and after cleaning the residues of bacteria were assayed with the ATP bioluminescenee and bacteria counting method for parallel comparison. Results After cleaning drying program, the RLU of two groups in suction head internal top,2.5em at inside straw joint,the front of straw outer wall, 10em at suction cavity wall had significant differences （t = 26.81, 29. 13,7.14,55.74 ,P 〈 0.01 ）. The cleaning effect of B group was better than A group.. Bacteria counting method was used to detect pathogenic microorganism that was not detected. The qualified rate of cleaning, at four parts was as following： group A ：70.0% ,85.0% ,93.3% ,93.3%. B group ：95.0% ,98.3%, 100.0%, 100.0%, and the quali- fied rate of group B was significantly higher than group A （ X2 = 12. 99,6. 98,4. 14,4. 14, all P 〈 0.05 ）. Conclusion ATP biolumineseenee method can dynamically rapid detect the bacteria before and after intrauterine suction cleaning residues, can be viewed as an effective method to detect quality of uterine suction cleaning.

关 键 词：荧光素酶法 细菌 外科器械 质量控制 

分 类 号：R187[医药卫生—流行病学]