维药瘤果黑种草子总皂苷镇咳祛痰及抗炎作用研究  

A study on antitussive,expectorant and anti-inflammatory effects of total saponins from Uyghur Herb Nigella glandulifera freyn

作　　者：耿东升[1] 赵丽萍[1] 史荣梅[1] 陈雪莲[1] 

机构地区：[1]新疆军区联勤部药品仪器检验所,乌鲁木齐830063

出　　处：《新疆医科大学学报》2013年第7期908-911,共4页Journal of Xinjiang Medical University

基　　金：国家自然科学基金(30960512)

摘　　要：目的研究探讨维药瘤果黑种草子总皂苷的镇咳、祛痰和抗炎作用。方法选择昆明种小鼠及健康新西兰种家兔为实验动物,采用小鼠浓氨水引咳法观察总皂苷的镇咳作用,小鼠气管苯酚红排泌法、家兔促进气管纤毛粘液实验法观察总皂苷的祛痰作用,二甲苯致小鼠耳廓肿胀、角叉菜胶致小鼠足跖肿胀实验观察总皂苷的抗炎作用。结果总皂苷可明显延长小鼠咳嗽的潜伏期,其高剂量组可显著降低氨水致小鼠的咳嗽次数(P<0.01);总皂苷能明显增加小鼠气管酚红排泌量(P<0.05～0.01),增加家兔气管纤毛粘液流运动速度(P<0.01);总皂苷能明显抑制二甲苯所致的小鼠耳廓肿胀及角叉菜胶所致的足趾肿胀(P<0.01)。结论维药瘤果黑种草子总皂苷具有明显的镇咳、祛痰和抗炎作用。Objective To study the antitussive expectorant and anti-inflammatory effects of total saponins from Uyghur Herb Nigella glandulifera freyn. Methods KM mice and New Zealand rabbits were select-ed as experiment animals. The antitussive effect was studied by the cough model induced by ammonia in mice; the expectorant effect was studied by a mouse model of phenoled test and the rabbit tracheal eiliated mucous promote flow experiments; the anti-inflammatory effect was studied by the external ear swelling of mice induced by dimethyl benzene and the carragee-nan-induced paw edema in mice experiments. Results The experimental results showed that total saponins could prolong cough incubation period obviously, and the high dose group reduced the number of mice cough （P 〈0.01） ; increase phenol red excretion in mice （P 〈0. 05-0. 01） , increase rabbit tracheal eiliated mucous flow velocity （P 〈0.01）; inhibit dimethyl benzene-induced mouse ear and carragee-nan-induced mouse paw swelling degree （P 〈0.01）. Conclusion The total saponins from Uyghur Herb Nigella glandulifera freyn have obvious antitussive expectorant and anti-inflammatory effects.

关 键 词：瘤果黑种草子 总皂苷 镇咳作用 祛痰作用 抗炎作用 

分 类 号：R962[医药卫生—药理学] R974[医药卫生—药学]