金纳米棒非聚集比色法测定多巴胺  

Non-Aggregation Colorimetric Method for Determination of Dopamine

作　　者：郑志勇[1] 

机构地区：[1]漳州职业技术学院食品与生物工程系,福建漳州363000

出　　处：《分析测试技术与仪器》2013年第2期83-87,共5页Analysis and Testing Technology and Instruments

基　　金：漳州职业技术学院资助项目(ZZY1215)

摘　　要：基于在金纳米棒(AuNRs)-Ag+-多巴胺(DA)体系中,DA快速将Ag+还原为Ag,Ag包裹在AuNRs表面形成核壳状纳米棒(Au@AgNRs),改变了AuNRs周围的电介质环境,导致其纵横比减小、纵向等离子体共振吸收波长带(LPAB)蓝移,同时伴随着溶液的颜色发生显著的变化,藉此开发了一种快速测定DA的比色法.方法已成功应用于血清中DA的测定,其结果与荧光法相吻合.此外,探讨了比色法测定DA的机理.A non-aggregation colorimetric method for the rapid determination of dopamine （DA） has been developed based on the fact that DA can reduce Ag＋to Ag on the surface of gold nanorods （AuNRs） to form Au core-Ag shell nanorods （Au@ AgNRs） in a AuNRs-Ag＋- DA system, which leds to the change of dielectric function： the aspect ratio of AuNRs decreases, the longitudinal plasmon absorption band （LPAB） of AuNRs blue shifts （△λ ） , and the color of the solution changes obviously. The method has been successfully applied to the detection of DA in serum, and the results were in a good agreement with those determined by the fluorescence method. The mechanism for the detection of DA has also been discussed.

关 键 词：多巴胺 AuNRs纳米棒 比色法 

分 类 号：O655.2[理学—分析化学]