抗CATSPER1抗体对精子运动的影响  

The Influence of CATSPER1 Antibody to the Sperm Motility

作　　者：武文斌[1] 吉晓菲[2] 李玉山[2] 

机构地区：[1]郑州大学第三附属医院生殖中心,河南郑州450052 [2]郑州大学第三附属医院人类精子库,河南郑州450052

出　　处：《中国医药指南》2013年第16期472-474,共3页Guide of China Medicine

摘　　要：目的探讨CATSPER1蛋白在精子运动中的功能。方法将正常精液标本与终浓度分别为0.8μg/mL、4μg/mL、20μg/mL的CATSPER1抗体混合孵育,然后于37℃1h、2h、6h用计算机辅助分析系统(CASA)进行精液参数分析。结果在不同时间,前向运动精子(a+b级)、快速前向运动精子(a级)的百分比均较对照组明显减少并且成剂量依赖性,慢速前向运动精子(b级)受抗体抑制的作用不明显。结论 CATSPER1在精子运动过程中发挥重要功能,CATSPER1抗体主要抑制精子快速前向运动能力。Objective Objective： To evaluate the function of cation channel of sperm 1 （CATSPER1） protein in human sperm motility; Methods The sperm samples from normozoospermic donors were incucbated with different CATSPER1 antibody at final concentrations of 0,0.8,4,20μg/mL.Sperm motility was measured by means of computer-assisted semen analysis after 1, 2 and 6 hours incubation at 37℃. Results Progressive motility （class a＋b） and fast progressive motility （class a） of sperm were both inhibited under the use of CATSPER1 antibody in different concentrations and significant dose-dependent changes were observed （respectively, P〈0.01 and P〈0.05）and slow motility sperms were not inhibited at different time. Conclusion CATSPER1 protein plays an important role in maintaining the motility of mature sperm of which fast progressive motility can be inhibited by CATSPER1 antibody.

关 键 词：CATSPER1 精子运动 精液分析 

分 类 号：R698.2[医药卫生—泌尿科学]