A kind of modified bovine serum albumin with great potential for applying in gene delivery  

作　　者：Ning Zhang Chong-Xi Wang Jin-Hao Liu Jin-Feng Xing An-Jie Dong 

机构地区：[1]Department of Polymer Science and Engineering School of Chemical Engineering and Technology,Tianjin University

出　　处：《Chinese Chemical Letters》2013年第7期659-662,共4页中国化学快报（英文版）

基　　金：the financial support from National Natural Science Foundation of China(No.31100722);Tianjin Natural Science Foundation(No.13JCYBJC16500)

摘　　要：Bovine serum albumin （BSA） was modified through a facile synthesis method to increase its isoelectric point （pl） from 4.8 to 6.0. When pH is higher than 6.0, the protein shows a negative surface charge, on the contrary, the protein is positively charged. In this study, the charge-reversal modified BSA （crBgA） was utilized to assemble with the binary complexes of pDNA/poly（vinylpyrrolidone）-graft-poly（2- dimethylaminoethyl methacrylate） （pDNA/PVP-g-PDMAEMA） to shield the excess positive charges of complexes at physiological pH （pH 7.4）. When the complex coated with crBSA located in the environment at endosomal pH （pH 5.0）, the charge-reversal of crBSA led to the deviation of crBSA from polyplex by electrostatic repulsion, which would benefit the transfection of the target gene. The crBSA shows great potential for improving the transfection efficiency of ~DNA/PVP-^-PDMAEMA.Bovine serum albumin （BSA） was modified through a facile synthesis method to increase its isoelectric point （pl） from 4.8 to 6.0. When pH is higher than 6.0, the protein shows a negative surface charge, on the contrary, the protein is positively charged. In this study, the charge-reversal modified BSA （crBgA） was utilized to assemble with the binary complexes of pDNA/poly（vinylpyrrolidone）-graft-poly（2- dimethylaminoethyl methacrylate） （pDNA/PVP-g-PDMAEMA） to shield the excess positive charges of complexes at physiological pH （pH 7.4）. When the complex coated with crBSA located in the environment at endosomal pH （pH 5.0）, the charge-reversal of crBSA led to the deviation of crBSA from polyplex by electrostatic repulsion, which would benefit the transfection of the target gene. The crBSA shows great potential for improving the transfection efficiency of ~DNA/PVP-^-PDMAEMA.

关 键 词：Bovine serum albumin Gene delivery Charge reversal Polyplexes 

分 类 号：Q51[生物学—生物化学]