熊果酸对神经母细胞瘤细胞增殖凋亡及MYCN表达的影响  

Ursolic acid effects proliferation .apoptosis and MYCN expression of neuroblastoma cell.

作　　者：邢莉莉[1] 吴晔明[1] 张弛[1] 

机构地区：[1]上海交通大学医学院附属新华医院,上海市200092

出　　处：《临床小儿外科杂志》2013年第3期196-200,共5页Journal of Clinical Pediatric Surgery

摘　　要：目的实验研究熊果酸作用于体外培养的神经母细胞瘤细胞株（SH—SY-5Y），对肿瘤细胞增殖、凋亡及MYCN基因mRNA表达的影响，探讨熊果酸对儿童神经母细胞瘤的治疗的作用。方法以终浓度为4uM／L、8uM／L、12uM／L的熊果酸作用于神经母细胞瘤细胞SH—SY-5Y，WST-1法检测SH—SY-5Y细胞增殖情况，流式细胞术检测SH—SY-5Y细胞凋亡情况，采用RealtimePCR方法检测MYCNmRNA的表达情况。结果熊果酸对SH—SY-5Y细胞增殖的抑制呈明显的剂量、时间依赖性，随剂量的增加，作用时间的延长，抑制效果逐渐加强；熊果酸对SHSY-5Y细胞凋亡的促进呈明显的剂量依赖性，随剂量的增加，促进凋亡效果逐渐加强；熊果酸对SH—SY-5Y细胞MYCN表达有抑制作用并有明显的剂量依赖性，随剂量的增加，抑制效果逐渐加强。12uM／L作用72h后神经母细胞瘤细胞增殖抑制效率达98．3％，MYCN基因mRNA表达抑制率达52．37％。结论应用熊果酸在体外环境作用SH—SY-5Y细胞，可以有效抑制该细胞的增殖、促进细胞凋亡、抑制原癌基因MYCNmRNA的表达。Objetive This study was designed to investigate the effects of ursolic acid in proliferation, apoptosis and MYCN gene mRNA expression of SH-SY-5Y cell line cultured in vitro, in order to investigate the effect of ursolic acid （UA） in neuroblastoma（NB）. Method Neuroblastoma SH-SY-5Y cells were treated by 4 uM/L,8 uM/L,12 uM/L ursolic acid. WST-I was used to measure the levels of the proliferation of SH-SY- 5Ycells cultured with different concentrations of ursolie acid. While SH-SY-SYcells apoptosis was measured by flow cytometry. MYCN gene mRNA expression in SH-SY-SYceils was measured using real time PCR. Results The prollferation of SH-SY-5Ycells was obviously inhibited by ursolic acid in a dose-and time-dependent man- net. The apoptosis of SH-SY-5Ycells was obviously promoted hy ursolic acid in a dose-dependent manner. The MYCN gene mRNA expression in SH-SY-SYcells was obviously inhibited by ursolic acid in a dose-ependent manner. Ursolic acid of 12 uM/L treatment for 72 hrs produced a best effect with an inhibitory rate of SH-SY- 5Y cell proliferation of 98.3 % and inhibitory rate of MYCN gene mRNA expression of 52.37%. Conclu- sions Ursolic acid may inhibit tile growth of neuroblastoma ceils, promote neuroblastoma cells apoptosis and down-regulate MYCN mRNA expression.

关 键 词：神经母细胞瘤 熊果酸 细胞增殖 细胞凋亡 MYCN 

分 类 号：R114[医药卫生—卫生毒理学]