疏风通络方对哮喘大鼠肺组织嗜酸细胞浸润影响随机平行对照研究  

A Randomized and Parallel - controlled Study of Influnce of Shufeng Tongluo Decoction on Eosinophil Infiltration in Lung of Asthmatic Model Rats

作　　者：张慧琪[1] 刘贵颖[1] 尹新中[1] 朱振刚[1] 刘旻[1] 乔亚珍[1] 窦颖婷[1] 刘文彬[1] 

机构地区：[1]天津中医药大学第一附属医院,天津南开300193

出　　处：《实用中医内科杂志》2013年第3期76-79,共4页Journal of Practical Traditional Chinese Internal Medicine

基　　金：2009国家自然科学基金项目(No:30973727)

摘　　要：[目的]观察疏风通络方对哮喘大鼠肺组织嗜酸细胞浸润影响。[方法]使用随机平行对照方法,将SPF级雄性Wistar大鼠70只按随机数字表方法随机分为7组,10只/组,分笼饲养,标记实验组别和实验开始日期。复制哮喘大鼠模型后,分别进行干预,实验第1d各组大鼠致敏。正常组生理盐水1mL腹腔注射;其他六组1mL造模液(含Ⅴ级卵清蛋白100mg,氢氧化铝100mg和灭活百日咳杆菌6×109个)腹腔注射。第15d开始激发:各组大鼠分别置于相同大小雾化箱内,正常组生理盐水6mL雾化,其他六组5%Ⅴ级卵清蛋白(OVA)溶液6mL雾化。每次激发前2h灌胃:正常组生理盐水1mL,其他六组分别给予相应药物灌胃,模型组生理盐水1mL;激素组氟美松按0.5mg/kg体重;DPI组DPI 5μmoL/kg体重,中药低、中、高剂量组10.6g/kg、21.2g/kg、42.4g/kg。连续10d激发后用3%戊巴比妥钠腹腔注射(50mg/kg)麻醉大鼠,麻醉下待大鼠反应迟钝时将其固定于解剖台上,打开胸腔,暴露心肺。迅速取左肺组织,截取多个部位的肺组织标本分别置于中性甲醛固定液中固定以便制作病理切片。心脏取血,血标本另作其他指标检测;结扎左肺主支气管,生理盐水灌洗右肺主支气管,回收的肺泡灌洗液留作其他指标检测用。观测肺组织嗜酸细胞形态。[结果]模型组肺组织嗜酸细胞浸润最为显著,明显高于正常组;各治疗组与模型组相比,地塞米松组肺组织嗜酸细胞浸润程度最轻,中药高剂量组次之,其余组嗜酸细胞浸润减轻不显著。[结论]疏风通络方能减轻哮喘大鼠肺组织嗜酸细胞浸润,高剂量组更显著,这可能是其平喘作用机制之一。[Objective]To explore Influnce of Shufeng Tongluo decoction on eosinophil infiltration in lung of asthmatic model rats.[Method]Seventy Wistar Rats（SPF） were randomly divided into seven groups according to random number table as follows： normal group,model group,Dexamethasone group,DPI group,low-dose group of Chinese medicine,medium-dose group of Chinese medicine,and high-dose group of Chinese medicine,10 rats per group.The rats in normal group were sensitized by intraperitoneally injected normal saline 1ml and ones in other 6 groups by 1mL sensitization liquid which contained 100mg OVA（Grade V）,100mg aluminum hydroxide and 6 × 109Inactivated whooping cough bacili on the first day of experimental study.From 15th day on,The rats in normal group were administrated normal saline 1ml by oral way and 2 hours later were challenged by 6mL normal saline aerosol inhalation once a day,30min a time,keeping for 10 days;the rats in other 6 group were respectively administrated corresponding drugs by oral way and 2 hours later were challenged by 6mL OVA（5%,V Grade） aerosol inhalation once a day,30min a time,keeping for 10 days.10 days later lung tissue samples from rats were collected under anesthesia,samples were fixed in 10% formalin,and then made pathological sections,observed them under a microscope.[Result]The eosinophil infiltration in model group is most significant,and higher than that of normal group;compared with the model group,Dexamethasone group can significantly decreased teosinophil infiltration,effect of high-dose group was inferior to Dexamethasone group,and the other treatment groups only had general inhibition effect.[Conclusion]Shufeng Tongluo decoction could inhibit eosinophil infiltration in lung of asthmatic model rats to a certain extent,high-dose group was more significant,as may be one of its action mechanisms of controlling asthma.

关 键 词：哮喘大鼠模型 肺组织嗜酸细胞浸润 疏风通络方 氟美松片 氯化二联苯碘 随机平行对照研究 

分 类 号：R285.5[医药卫生—中药学]