高效液相色谱法测定虎杖中白藜芦醇和白藜芦醇苷的含量  

Determination of Content Resveratrol and Polydatin from Polygonum Cuspidatum by HPLC

作　　者：邢琪昌[1] 卢昊[1] 付晓[2] 李力[1] 成巧[1] 许汉林[1] 

机构地区：[1]湖北中医药大学药学院,湖北武汉430065 [2]三峡大学,湖北宜昌443002

出　　处：《湖北中医药大学学报》2013年第3期33-35,共3页Journal of Hubei University of Chinese Medicine

基　　金：湖北省科技厅科学技术项目(2005AA301C32);武汉市科学技术局科学技术研究项目(201060623269)

摘　　要：目的测定虎杖中白藜芦醇和白藜芦醇苷的含量。方法采用高效液相色谱法测定,色谱柱为LichrospherC18,流动相为乙腈-0.03%磷酸水溶液(30∶70),梯度洗脱,流速:1.0 mL/min,柱温:30℃,检测波长:306nm进样量20mL。结果白藜芦醇的回归方程为:Y=83.7120X+2501.7909,r=0.8351。白藜芦醇在41.8-10.22×103μg含量范围内线性关系良好;白藜芦醇苷的回归方程为:Y=89.5637X+1045.3914,r=0.8324。白藜芦醇苷在109.056-4260μg含量范围内线性关系良好。结论该法准确、简便、重现性好,适用于虎杖中白藜芦醇和白藜芦醇苷的定量分析。Objectve Determination of Resveratrol and Polygonum cuspidatum polydatin content. Methods Determination of the col- umn Lichrospher CTM, mobile phase： 0.03% phosphoric acid solution （30： 70）, the gradient elution flow rate of 1.0 mL/min. Temperature of 30℃, the detection wavelength UV. Results Resveratrol regression equation ： Y = 83. 7120X ＋ 2501. 7909,r =0. 8351. in 41.8-10.22×103 μg range showed good linear relationship. Polydatin the regression equation ： Y = 89. 5637X ＋ 1045. 3914, r = 0. 8324. in 109. 056 - 4260 μg range showed good linear realationship. Conclusion This method is accurate, reproducible and applicable to Polygonum cuspidatum resveratrol and polydatin quantitative analysis.

关 键 词：虎杖 白藜芦醇 白藜芦醇苷 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]