血管生长因子在血吸虫病大肠癌患者肠组织中的表达  

Expression of vascular growth factors in intestinal tissues in colorectal carci noma patients with schistosomiasis japonica

作　　者：阮水良[1] 王波[1] 陆其明[1] 董来荣[1] 曹晨羲[1] 徐水凌[1] 沈文艳[1] 

机构地区：[1]嘉兴学院附属第二医院,嘉兴314000

出　　处：《中国血吸虫病防治杂志》2013年第3期250-254,共5页Chinese Journal of Schistosomiasis Control

基　　金：浙江省嘉兴市科技局资助项目(2010AY1037)

摘　　要：目的探讨血管生长因子在有血吸虫病和无血吸虫病的大肠癌患者肠组织中的mRNA和蛋白表达差异。方法收集30例有血吸虫病及30例无血吸虫病大肠癌患者的大肠癌手术切除标本中的正常肠组织、癌旁组织和癌组织,采用荧光实时定量PCR和Western blot检测不同组织中血小板衍生内皮细胞生长因子(PD-ECGF)和血管内皮生长因子(VEGF)的mRNA和蛋白表达水平。结果有血吸虫病大肠癌患者正常组织、癌旁组织及癌组织中PD-ECGF mRNA和VEFG mRNA相对表达量是无血吸虫病患者的1.726、1.766、2.729倍和2.138、1.831、3.376倍,有血吸虫病大肠癌患者正常组织、癌旁组织及癌组织中PD-ECGF和VEGF蛋白表达水平均显著高于对应的无血吸虫病患者肠组织中的表达水平(P均<0.05)。结论血管生长因子PD-ECGF和VEGF在有血吸虫病大肠癌患者肠组织中的表达水平高于无血吸虫病患者,血吸虫病可能是大肠癌发病的高危因素之一。Objective To investigate the differences of mRNA quantitation and protein expression of vascular growth factors including platelet-derived endothelial cell growth factor（PD-ECGF）and vascular endothelial growth factor（VEGF）in intestinal tissues in colorectal carcinoma patients with and without schistosomiasis.Methods Thirty colorectal carcinoma patients with schistosomiasis and 30 colorectal carcinoma patients without schistosomiasis were included in this study.The mRNA quantitation and protein expression of PD-ECGF and VEGF in the normal tissue,peri-carcinoma tissue as well as carcinoma tissue obtained from surgical specimens were detected by qRT-PCR and Western blot.Results The mRNA relative quantitations of PD-ECGF in normal tissue,peri-carcinoma tissue and carcinoma tissue in the colorectal carcinoma patients with schistosomiasis were 1.726,1.766 and 2.729 times to those in the colorectal carcinoma patients without schistosomiasis,respectively.The correspond ing ones of VEGF were 2.138,1.831 and 3.376 times,respectively.The protein expression levels of PD-ECGF and VEGF in nor mal tissue,peri-carcinoma tissue and carcinoma tissue were higher in the colorectal carcinoma patients with schistosomiasis than in the colorectal carcinoma patients without schistosomiasis.Conclusions The expressions of vascular growth factors including PD-ECGF and VEGF are higher in the colorectal carcinoma patients with schistosomiasis than in the colorectal carcinoma patients without schistosomiasis.Therefore,schistosomiasis may be one of the risk factors of colorectal cancer.

关 键 词：日本血吸虫病 大肠癌 血小板衍生内皮细胞生长因子 血管内皮生长因子 

分 类 号：R532.21[医药卫生—内科学]